ArticleApplied and environmental microbiology2010
Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
89 citing papers in PubMed, 1 synthesis or guideline pooled it, 213 citations in OpenAlex.
- Linking genome content to biofuel production yields: a meta-analysis of major catabolic pathways among select H2 and ethanol-producing bacteria.BMC microbiology · 2012Pooled it
- Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg.Journal of biological engineering · 2025Article
- Thermophilic site-specific recombination system for rapid insertion of heterologous DNA into the Clostridium thermocellum chromosome.Journal of industrial microbiology & biotechnology · 2024Article
- Global trends and future prospects of lactic acid production from lignocellulosic biomass.RSC advances · 2023Article
- Metabolic engineering of Thermoanaerobacterium AK17 for increased ethanol production in seaweed hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- Base editor enables rational genome-scale functional screening for enhanced industrial phenotypes inScience advances · 2022Article
- A Single Nucleotide Change in theApplied and environmental microbiology · 2022Article
- Unifying themes and distinct features of carbon and nitrogen assimilation by polysaccharide-degrading bacteria: a summary of four model systems.Applied microbiology and biotechnology · 2021Review
- Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose.Environmental microbiology reports · 2021Review
- Development of aJournal of Zhejiang University. Science. B · 2021Article
- Thermophilic whole-cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum.Microbial biotechnology · 2021Article
- Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles.The CRISPR journal · 2021Article
- Approaches to genetic tool development for rapid domestication of non-model microorganisms.Biotechnology for biofuels · 2021Review
- Development of both type I-B and type II CRISPR/Cas genome editing systems in the cellulolytic bacteriumMetabolic engineering communications · 2020Article
- The virulence factor urease and its unexplored role in the metabolism of Cryptococcus neoformans.FEMS yeast research · 2020Article
- CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313.Microbial biotechnology · 2020Article
- Synthetic Biology Tools for Genome and Transcriptome Engineering of SolventogenicFrontiers in bioengineering and biotechnology · 2020Review
- Synthetic Biology and Metabolic Engineering EmployingFrontiers in bioengineering and biotechnology · 2020Review
- Article
- Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems.Journal of industrial microbiology & biotechnology · 2019Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene. The ΔpyrF strain is a uracil auxotroph that could be restored to a prototroph via ectopic expression of pyrF from a plasmid, providing a positive genetic selection. Furthermore, 5-FOA was used to select against plasmid-expressed pyrF, creating a negative selection for plasmid loss. This technology was used to delete a gene involved in organic acid production, namely pta, which encodes the enzyme phosphotransacetylase. The C. thermocellum Δpta strain did not produce acetate. These results are the first examples of targeted homologous recombination and metabolic engineering in C. thermocellum, a microbe that holds an exciting and promising future in the biofuel industry and development of sustainable energy resources.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.