ArticleJournal of experimental botany2010
Functional analysis of the structural domain of ARF proteins in rice (Oryza sativa L.).
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 173 citations in OpenAlex.
- Pan-genome analysis reveals hidden diversity and selection signatures of auxin response factors (ARFs) associated with breeding in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- An auxin response factor regulates tiller angle and shoot gravitropism by directly activating related gene expression in rice.Journal of advanced research · 2025Article
- Comprehensive Analysis of the ARF Gene Family Reveals Their Roles in Chinese Chestnut (Biology · 2025Article
- The Auxin Response FactorPlants (Basel, Switzerland) · 2025Article
- Bioinformatics Analysis Reveals the Evolutionary Characteristics of theInternational journal of molecular sciences · 2025Article
- A conserved ARF-DNA interface underlies auxin-triggered transcriptional response.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Functional Divergence of the Closely Related Genes PhARF5 and PhARF19a inInternational journal of molecular sciences · 2024Article
- Genome-Wide Identification, Expression, and Interaction Analysis of the Auxin Response Factor andInternational journal of molecular sciences · 2024Article
- The Receptor Kinases DRUS1 and DRUS2 Behave Distinctly in Osmotic Stress Tolerance by Modulating the Root System Architecture via Auxin Signaling.Plants (Basel, Switzerland) · 2024Article
- Heat Stress Responsive Aux/IAA Protein, OsIAA29 Regulates Grain Filling Through OsARF17 Mediated Auxin Signaling Pathway.Rice (New York, N.Y.) · 2024Article
- The Potential Role of Genic-SSRs in Driving Ecological Adaptation Diversity inInternational journal of molecular sciences · 2024Article
- Genomic survey and expression analysis of LcARFs reveal multiple functions to somatic embryogenesis in Liriodendron.BMC plant biology · 2024Article
- Game of thrones among AUXIN RESPONSE FACTORs-over 30 years of MONOPTEROS research.Journal of experimental botany · 2023Review
- Genome-Wide Identification and Expression Analysis of Auxin Response Factor (ARF) Gene Family inPlants (Basel, Switzerland) · 2023Article
- Analysis of Auxin-Encoding Gene Family inPlants (Basel, Switzerland) · 2023Article
- Unraveling the Guardians of Growth: A Comprehensive Analysis of thePlants (Basel, Switzerland) · 2023Article
- Recent advances in auxin biosynthesis and homeostasis.3 Biotech · 2023Review
- Genome-Wide Identification and Expression Analysis of Auxin Response Factor Gene Family inInternational journal of molecular sciences · 2023Article
- Cooperative action of separate interaction domains promotes high-affinity DNA binding ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The identification and characterization of a plant height and grain length related geneFrontiers in plant science · 2023Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auxin response factors (ARFs) are key regulators of plant growth and development. Through interaction with auxin/indole acetic acid (Aux/IAA) proteins, they influence the expression of auxin response genes. An ARF gene family has been predicted in rice, but the functions of the individual structural domains of the OsARFs remain obscure. Bioinformatics was used to analyse the position of the DNA-binding domain (DBD), middle region (MR), and C-terminal dimerization domain (CTD) of OsARFs, and experimentally confirmed the presence of a classical monopartite nuclear localization signal (NLS) in the DBD. The DBD was shown to contribute to nuclear localization of OsARF proteins in addition to its known DNA-binding function. Interactions between 14 integrated OsARFs and 15 OsIAA proteins were tested using yeast two-hybrid assays. It was found that eight OsARF activators interacted with the 15 OsIAA proteins, while six OsARF repressors did not. The interactions between the MR+CTD or CTD of 10 OsARFs and 15 OsIAA proteins were also tested and the results were consistent with those of each intact OsARF, although some slight differences in interaction intensity were observed by α-galactosidase quantitative assays. The truncated CTD of OsARF11 did not interact with any OsIAA, implying that the CTD is required for ARF-IAA dimerization, and that the MR influences the interaction intensity in yeast. A subset of the interactions in yeast were also observed in tobacco plants using firefly luciferase complementation imaging assays, indicating that these interactions are specific in plants, and might have a special role in the auxin signalling response. This study provides new insight into the structure of OsARF proteins and ARF-Aux/IAA interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.