ArticleJournal of bacteriology2010
Bright mutants of Vibrio fischeri ES114 reveal conditions and regulators that control bioluminescence and expression of the lux operon.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- The conserved global regulator H-NS has a strain-specific impact on biofilm formation inbioRxiv : the preprint server for biology · 2024Article
- Quorum sensing inhibits interference competition among bacterial symbionts within a host.Current biology : CB · 2023Article
- Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications.Life (Basel, Switzerland) · 2022Article
- Nocturnal Acidification: A Coordinating Cue in theInternational journal of molecular sciences · 2022Review
- Article
- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
- Control of Competence in Vibrio fischeri.Applied and environmental microbiology · 2021Article
- Quorum Sensing and Cyclic di-GMP Exert Control Over Motility ofFrontiers in microbiology · 2021Article
- Multiplexed Competition in a Synthetic Squid Light Organ Microbiome Using Barcode-Tagged Gene Deletions.mSystems · 2020Article
- Genetic Manipulation of Vibrio fischeri.Current protocols in microbiology · 2020Article
- Ambient pH Alters the Protein Content of Outer Membrane Vesicles, Driving Host Development in a Beneficial Symbiosis.Journal of bacteriology · 2019Article
- Acidic pH promotes lipopolysaccharide modification and alters colonization in a bacteria-animal mutualism.Molecular microbiology · 2019Article
- Tools for Rapid Genetic Engineering of Vibrio fischeri.Applied and environmental microbiology · 2018Article
- An Iterative, Synthetic Approach To Engineer a High-Performance PhoB-Specific Reporter.Applied and environmental microbiology · 2018Article
- Comparative analysis reveals regulatory motifs at the ainS/ainR pheromone-signaling locus of Vibrio fischeri.Scientific reports · 2017Article
- An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.Applied and environmental microbiology · 2017Article
- Rethinking the roles of CRP, cAMP, and sugar-mediated global regulation in the Vibrionaceae.Current genetics · 2016Review
- Antisocial luxO Mutants Provide a Stationary-Phase Survival Advantage in Vibrio fischeri ES114.Journal of bacteriology · 2015Article
- Regulation of Bioluminescence in Photobacterium leiognathi Strain KNH6.Journal of bacteriology · 2015Article
- Modeling Analysis of Signal Sensitivity and Specificity by Vibrio fischeri LuxR Variants.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Vibrio fischeri ES114, an isolate from the Euprymna scolopes light organ, produces little bioluminescence in culture but is ∼1,000-fold brighter when colonizing the host. Cell-density-dependent regulation alone cannot explain this phenomenon, because cells within colonies on solid medium are much dimmer than symbiotic cells despite their similar cell densities. To better understand this low luminescence in culture, we screened ∼20,000 mini-Tn5 mutants of ES114 for increased luminescence and identified 28 independent "luminescence-up" mutants with insertions in 14 loci. Mutations affecting the Pst phosphate uptake system led to the discovery that luminescence is upregulated under low-phosphate conditions by PhoB, and we also found that ainS, which encodes an autoinducer synthase, mediates repression of luminescence during growth on plates. Other novel luminescence-up mutants had insertions in acnB, topA, tfoY, phoQ, guaB, and two specific tRNA genes. Two loci, hns and lonA, were previously described as repressors of bioluminescence in transgenic Escherichia coli carrying the light-generating lux genes, and mutations in arcA and arcB were consistent with our report that Arc represses lux. Our results reveal a complex regulatory web governing luminescence and show how certain environmental conditions are integrated into regulation of the pheromone-dependent lux system.
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