ArticleThe Journal of physiology2010
Modulation of single-channel properties of TRPV1 by phosphorylation.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 83 citations in OpenAlex.
- Inhibition of Microglial TRPV1 Ameliorates Brain Injury After Intracerebral Hemorrhage by Suppressing AMPK/PINK1-Mediated Mitophagy.CNS neuroscience & therapeutics · 2026Article
- Tyrosine phosphorylation and palmitoylation of TRPV2 ion channel tune microglial beta-amyloid peptide phagocytosis.Journal of neuroinflammation · 2024Article
- The dual role of TRPV1 in peripheral neuropathic pain: pain switches caused by its sensitization or desensitization.Frontiers in molecular neuroscience · 2024Review
- Diacylglycerol Activates theInternational journal of molecular sciences · 2023Article
- A journey from molecule to physiology andFrontiers in pharmacology · 2023Review
- Article
- PKC regulation of ion channels: The involvement of PIPThe Journal of biological chemistry · 2022Review
- TRPV1 channels as a newly identified target for vitamin D.Channels (Austin, Tex.) · 2021Review
- Activation of Oxytocin Receptors Excites Subicular Neurons by Multiple Signaling and Ionic Mechanisms.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Th2 Modulation of Transient Receptor Potential Channels: An Unmet Therapeutic Intervention for Atopic Dermatitis.Frontiers in immunology · 2021Review
- Kainic Acid Activates TRPV1 via a Phospholipase C/PIP2-Dependent Mechanism in Vitro.ACS chemical neuroscience · 2020Article
- Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel.The Journal of physiology · 2020Article
- Effect of 158 herbal remedies on human TRPV1 and the two-pore domain potassium channels KCNK2, 3 and 9.Journal of traditional and complementary medicine · 2020Article
- β-Arrestin 2 and ERK1/2 Are Important Mediators Engaged in Close Cooperation between TRPV1 and µ-Opioid Receptors in the Plasma Membrane.International journal of molecular sciences · 2020Article
- Structural and Evolutionary Insights Point to Allosteric Regulation of TRP Ion Channels.Accounts of chemical research · 2019Article
- Bone Metastasis Pain, from the Bench to the Bedside.International journal of molecular sciences · 2019Review
- TRPV1 pore turret dictates distinct DkTx and capsaicin gating.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Interactions of the immune and sensory nervous systems in atopy.The FEBS journal · 2018Review
- Article
- Magnetothermal genetic deep brain stimulation of motor behaviors in awake, freely moving mice.eLife · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Activation of TRPV1, the heat and capsaicin receptor, is known to be promoted by phosphorylation, but the molecular details are unclear. In the present study we recorded from single TRPV1 ion channels using the cell-attached patch clamp technique. The influence of capsaicin concentration on the time constants of open and closed states demonstrates the existence of at least four closed and three open states, and shows that channel opening can occur from partially liganded states. Activation of protein kinase C (PKC) promotes channel opening in some channels but not others, consistent with some channels being inaccessible to the kinase. The changes in open and closed state time constants following activation of PKC are equivalent to an increased affinity of capsaicin binding, but other arguments suggest that channel opening must be potentiated by downstream changes in channel activation rather than by a direct action of phosphorylation on the capsaicin binding site. Mutation of functionally important PKC phosphorylation sites on TRPV1, or application of staurosporine, a broad-spectrum kinase inhibitor, completely inhibited the effect of PKC in enhancing channel open time. Staurosporine also inhibited channel activity in the absence of overt PKC activation, showing that TRPV1 is partially phosphorylated at rest. This study elucidates the mechanism by which phosphorylation by PKC potentiates the activation of single TRPV1 ion channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.