ArticleCurrent biology : CB2010
Sequential activation of apical and basolateral contractility drives ascidian endoderm invagination.
Article in Current biology : CB, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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108 citing papers in PubMed, 223 citations in OpenAlex.
- Article
- Actomyosin contractility drives apical polarization and membrane transport during tubulogenesis.Research square · 2026Article
- Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing.Nature communications · 2025Article
- Application and measurement of tissue-scale tension in avian epithelia in vivo to study multiscale mechanics and inter-germ layer coupling.Development (Cambridge, England) · 2025Article
- A multi-tiered mechanical mechanism shapes the early neural plate.Nature communications · 2025Article
- Article
- Physics of notochord tube expansion in ascidians.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation.eLife · 2025Article
- Internal cohesion gradient as a novel mechanism of collective cell migration.PLoS computational biology · 2025Article
- A Morphospace Exploration Using a General Model of Development Reveals a Basic Set of Morphologies for Early Animal Development and Evolution.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- The mechanical influence of densification on epithelial architecture.PLoS computational biology · 2024Article
- Inter-plane feedback coordinates cell morphogenesis and maintains 3D tissue organization in the Drosophila pupal retina.Development (Cambridge, England) · 2024Article
- Mechanical regulation of substrate adhesion and de-adhesion drives a cell-contractile wave during Drosophila tissue morphogenesis.Developmental cell · 2024Article
- Embryo mechanics cartography: inference of 3D force atlases from fluorescence microscopy.Nature methods · 2023Article
- Two-point optical manipulation reveals mechanosensitive remodeling of cell-cell contacts in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development and Application of an Optogenetic Manipulation System to Suppress Actomyosin Activity inInternational journal of molecular sciences · 2023Article
- Curvature gradient drives polarized tissue flow in theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Homeotic compartment curvature and tension control spatiotemporal folding dynamics.Nature communications · 2023Article
- Lmo7 recruits myosin II heavy chain to regulate actomyosin contractility and apical domain size in Xenopus ectoderm.Development (Cambridge, England) · 2022Article
- Force-generating apoptotic cells orchestrate avian neural tube bending.Developmental cell · 2022Article
48 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundEpithelial invagination is a fundamental morphogenetic behavior that transforms a flat cell sheet into a pit or groove. Previous studies of invagination have focused on the role of actomyosin-dependent apical contraction; other mechanisms remain largely unexplored.
resultsWe combined experimental and computational approaches to identify a two-step mechanism for endoderm invagination during ascidian gastrulation. During Step 1, which immediately precedes invagination, endoderm cells constrict their apices because of Rho/Rho-kinase-dependent apical enrichment of 1P-myosin. Our data suggest that endoderm invagination itself occurs during Step 2, without further apical shrinkage, via a novel mechanism we call collared rounding: Rho/Rho-kinase-independent basolateral enrichment of 1P-myosin drives apico-basal shortening, whereas Rho/Rho-kinase-dependent enrichment of 1P and 2P myosin in circumapical collars is required to prevent apical expansion and for deep invagination. Simulations show that boundary-specific tension values consistent with these distributions of active myosin can explain the cell shape changes observed during invagination both in normal embryos and in embryos treated with pharmacological inhibitors of either Rho-kinase or Myosin II ATPase. Indeed, we find that the balance of strong circumapical and basolateral tension is the only mechanism based on differential cortical tension that can explain ascidian endoderm invagination. Finally, simulations suggest that mesectoderm cells resist endoderm shape changes during both steps, and we confirm this prediction experimentally.
conclusionsOur findings suggest that early ascidian gastrulation is driven by the coordinated apposition of circumapical and lateral endoderm contraction, working against a resisting mesectoderm. We propose that similar mechanisms may operate during other invaginations.
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