ArticleBiochimica et biophysica acta2010
Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Impact of pulse duration on cardiac electroporation: nanosecond pulses enhance cardiomyocyte selectivity and promote a Raman-detected shift towards apoptotic cell death.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2025Article
- Potentiation of Gelonin Cytotoxicity by Pulsed Electric Fields.International journal of molecular sciences · 2025Article
- Ablating Myocardium Using Nanosecond Pulsed Electric Fields: Preclinical Assessment of Feasibility, Safety, and Durability.Circulation. Arrhythmia and electrophysiology · 2024Article
- Pulsed Field Ablation for the Treatment of Atrial Fibrillation: A Review and a Look into its Future.The heart surgery forum · 2024Article
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- Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP).International journal of molecular sciences · 2023Article
- Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study.International journal of molecular sciences · 2023Article
- Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?Annals of medicine · 2022Article
- Pulse width and intensity effects of pulsed electric fields on cancerous and normal skin cells.Scientific reports · 2022Article
- Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora's Box.International journal of molecular sciences · 2022Review
- How to alleviate cardiac injury from electric shocks at the cellular level.Frontiers in cardiovascular medicine · 2022Article
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- Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells.Scientific reports · 2019Article
- Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors.Scientific reports · 2019Article
- Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks.Scientific reports · 2018Article
- Single Intense Microsecond Electric Pulse Induces Radiosensitization to Ionizing Radiation: Effects of Time Intervals Between Electric Pulse and Ionizing Irradiation.Frontiers in oncology · 2018Article
- Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric FieldsTechnology in cancer research & treatment · 2017Article
- Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects.The Journal of biological chemistry · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
backgroundNanosecond electric pulses (EP) disrupt cell membrane and organelles and cause cell death in a manner different from the conventional irreversible electroporation. We explored the cytotoxic effect of 10-ns EP (quantitation, mechanisms, efficiency, and specificity) in comparison with 300-ns, 1.8- and 9-μs EP.
methodsEffects in Jurkat and U937 cells were characterized by survival assays, DNA electrophoresis and flow cytometry.
results10-ns EP caused apoptotic or necrotic death within 2-20 h. Survival (S, %) followed the absorbed dose (D, J/g) as: S=alphaD((-K)), where coefficients K and alpha determined the slope and the "shoulder" of the survival curve. K was similar in all groups, whereas alpha was cell type- and pulse duration-dependent. Long pulses caused immediate propidium uptake and phosphatidylserine (PS) externalization, whereas 10-ns pulses caused PS externalization only.
conclusions1.8- and 9-μs EP cause cell death efficiently and indiscriminately (LD₅₀ 1-3 J/g in both cell lines); 10-ns EP are less efficient, but very selective (LD₅₀ 50-80 J/g for Jurkat and 400-500 J/g for U937); 300-ns EP show intermediate effects. Shorter EP open propidium-impermeable, small membrane pores ("nanopores"), triggering different cell death mechanisms. GENERAL SIGNIFICANCE: Nanosecond EP can selectively target certain cells in medical applications like tumor ablation.
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