Evidence map›Paper›PMID 20691222›Full record

ArticleJournal of microbiological methods2010

A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.

M Paula Goetting-Minesky, J Christopher Fenno

Abstract readEvaluation Study
In one paragraph

Article in Journal of microbiological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Genetic Manipulations of Oral Spirochete Treponema denticola.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  9. Approaches to Understanding Mechanisms of Dentilisin Protease Complex Expression inFrontiers in cellular and infection microbiology · 2021
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

M Paula Goetting-MineskyDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USA.
J Christopher Fenno

Funding

Surface protein complexes of oral treponemes: assembly and host cell interactionsR01DE018221 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FENNO, J CHRISTOPHER · 2009 to 2010
$752k
Choline phosphotransferase-dependent phospholipid synthesis in TreponemaR21AI079325 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FENNO, J CHRISTOPHER · 2008 to 2009
$418k
NIAID NIH HHS AI079325NIAID NIH HHS R21 AI079325NIDCR NIH HHS DE018221NIDCR NIH HHS R01 DE018221
6 · The paper itself

Abstract

The primary selectable marker for the genetic studies of Treponema denticola is a hybrid gene cassette containing both ermF and ermAM (ermB) genes. ErmB functions in Escherichia coli, while ErmF has been assumed to confer resistance in T. denticola. We demonstrate here that ErmB is sufficient for erythromycin selection in T. denticola and that the native ermB promoter drives ErmB expression.

Indexed as

Anti-Bacterial AgentsBacterial ProteinsDrug Resistance, BacterialErythromycinMutagenesisMutagenesis, InsertionalTreponema denticolaAnti-Bacterial AgentsBacterial ProteinsErythromycin

Identifiers

PMID20691222
PMCPMC2953711

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.