ArticleBMC microbiology2010
In Helicobacter pylori auto-inducer-2, but not LuxS/MccAB catalysed reverse transsulphuration, regulates motility through modulation of flagellar gene transcription.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2025Review
- Antimicrobial Peptides: A Promising Alternative to Conventional Antimicrobials for Combating Polymicrobial Biofilms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Molecular Mechanisms of Biofilm Formation inAntibiotics (Basel, Switzerland) · 2024Review
- Article
- Nanomaterials Regulate Bacterial Quorum Sensing: Applications, Mechanisms, and Optimization Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Biofilm formation and inhibition mediated by bacterial quorum sensing.Applied microbiology and biotechnology · 2022Review
- AI-2 Induces Urease Expression Through Downregulation of Orphan Response Regulator HP1021 inFrontiers in medicine · 2022Article
- Hesperetin Inhibits Expression of Virulence Factors and Growth ofInternational journal of molecular sciences · 2021Article
- Modulation of quorum sensing-associated virulence in bacteria: carbohydrate as a key factor.Archives of microbiology · 2021Review
- Biofilm Formation as a Complex Result of Virulence and Adaptive Responses ofPathogens (Basel, Switzerland) · 2020Review
- Proteomics ofMolecular & cellular proteomics : MCP · 2020Article
- Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli.Veterinary research · 2019Article
- Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance.Antimicrobial agents and chemotherapy · 2019Review
- Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference toFrontiers in microbiology · 2019Article
- Mechanisms of Bacterial Tolerance and Persistence in the Gastrointestinal and Respiratory Environments.Clinical microbiology reviews · 2018Review
- From deep-sea volcanoes to human pathogens: a conserved quorum-sensing signal in Epsilonproteobacteria.The ISME journal · 2015Article
- Biofilm Formation by Helicobacter pylori and Its Involvement for Antibiotic Resistance.BioMed research international · 2015Review
- A repetitive DNA element regulates expression of the Helicobacter pylori sialic acid binding adhesin by a rheostat-like mechanism.PLoS pathogens · 2014Article
- Factors that mediate colonization of the human stomach by Helicobacter pylori.World journal of gastroenterology · 2014Review
- Inhibitory effect of piperine on Helicobacter pylori growth and adhesion to gastric adenocarcinoma cells.Infectious agents and cancer · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundLuxS may function as a metabolic enzyme or as the synthase of a quorum sensing signalling molecule, auto-inducer-2 (AI-2); hence, the mechanism underlying phenotypic changes upon luxS inactivation is not always clear. In Helicobacter pylori, we have recently shown that, rather than functioning in recycling methionine as in most bacteria, LuxS (along with newly-characterised MccA and MccB), synthesises cysteine via reverse transsulphuration. In this study, we investigated whether and how LuxS controls motility of H. pylori, specifically if it has its effects via luxS-required cysteine metabolism or via AI-2 synthesis only.
resultsWe report that disruption of luxS renders H. pylori non-motile in soft agar and by microscopy, whereas disruption of mccAHp or mccBHp (other genes in the cysteine provision pathway) does not, implying that the lost phenotype is not due to disrupted cysteine provision. The motility defect of the DeltaluxSHp mutant was complemented genetically by luxSHp and also by addition of in vitro synthesised AI-2 or 4, 5-dihydroxy-2, 3-pentanedione (DPD, the precursor of AI-2). In contrast, exogenously added cysteine could not restore motility to the DeltaluxSHp mutant, confirming that AI-2 synthesis, but not the metabolic effect of LuxS was important. Microscopy showed reduced number and length of flagella in the DeltaluxSHp mutant. Immunoblotting identified decreased levels of FlaA and FlgE but not FlaB in the DeltaluxSHp mutant, and RT-PCR showed that the expression of flaA, flgE, motA, motB, flhA and fliI but not flaB was reduced. Addition of DPD but not cysteine to the DeltaluxSHp mutant restored flagellar gene transcription, and the number and length of flagella.
conclusionsOur data show that as well as being a metabolic enzyme, H. pylori LuxS has an alternative role in regulation of motility by modulating flagellar transcripts and flagellar biosynthesis through production of the signalling molecule AI-2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.