ArticleJournal of the American Chemical Society2010
Fluostatins produced by the heterologous expression of a TAR reassembled environmental DNA derived type II PKS gene cluster.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Genome mining and characterization of a heme-dependent enzyme catalyzing intermolecular Nitrogen-Nitrogen bond formation in hydrazinosuccinic acid biosynthesis.Synthetic and systems biotechnology · 2025Article
- Study about fluostatins: efficient preparation of fluostatin A and discovery of fluostatin derivatives from Streptomyces sp. TA-3391.The Journal of antibiotics · 2025Article
- Recent Developments and Challenges in the Enzymatic Formation of Nitrogen-Nitrogen Bonds.ACS catalysis · 2025Review
- Metabolic Blockade-Based Genome Mining of Sea Anemone-AssociatedMarine drugs · 2024Article
- Cofactor-independent C-C bond cleavage reactions catalyzed by the AlpJ family of oxygenases in atypical angucycline biosynthesis.Beilstein journal of organic chemistry · 2024Article
- Review
- Unravelling key enzymatic steps in C-ring cleavage during angucycline biosynthesis.Communications chemistry · 2023Article
- Heterologous expression of the crypticRSC advances · 2023Article
- Biochemical and structural insights of multifunctional flavin-dependent monooxygenase FlsO1-catalyzed unexpected xanthone formation.Nature communications · 2022Article
- Flavin-enabled reductive and oxidative epoxide ring opening reactions.Nature communications · 2022Article
- Isolation and identification of marine microbial products.Journal, genetic engineering & biotechnology · 2021Review
- Review
- Salumycin, a New Pyrazolequinone from aMolecules (Basel, Switzerland) · 2020Article
- Understand the Specific Regio- and Enantioselectivity of Fluostatin Conjugation in the Post-Biosynthesis.Biomolecules · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Iodine-mediated synthesis of benzo[RSC advances · 2019Article
- The functional differentiation of the post-PKS tailoring oxygenases contributed to the chemical diversities of atypical angucyclines.Synthetic and systems biotechnology · 2018Review
- Molecular basis of dimer formation during the biosynthesis of benzofluorene-containing atypical angucyclines.Nature communications · 2018Article
- HEx: A heterologous expression platform for the discovery of fungal natural products.Science advances · 2018Article
- Fluostatins M-Q Featuring a 6-5-6-6 Ring Skeleton and High Oxidized A-Rings from Marine Streptomyces sp. PKU-MA00045.Marine drugs · 2018Article
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3 authors.
Funding
Abstract
Culture independent approaches for accessing small molecules produced by uncultured bacteria are often hampered by the inability to easily clone environmental DNA (eDNA) fragments large enough to capture intact biosynthetic gene clusters that can be used in heterologous expression studies. Here we show that homology screening of eDNA megalibraries for clones containing natural product biosynthetic genes, coupled with transformation-assisted recombination (TAR) in yeast, can be used to access large, functionally intact, natural product gene clusters from the environment. The eDNA derived gene cluster reported here was functionally reconstructed from two overlapping cosmid clones using TAR. The isolation and structure elucidation of three new fluostatins (F, G, and H) produced by this TAR reconstructed gene cluster is described.
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