Evidence map›Paper›PMID 20689834›Full record

ArticlePloS one2010

Retinoic acids potentiate BMP9-induced osteogenic differentiation of mesenchymal progenitor cells.

Wenli Zhang, Zhong-Liang Deng, Liang Chen, Guo-Wei Zuo, Qing Luo, Qiong Shi, Bing-Qiang Zhang, Eric R Wagner, Farbod Rastegar, Stephanie H Kim and 17 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed, 128 citations in OpenAlex.

  1. Article
  2. Role of vitamins beyond vitamin DInternational journal of molecular medicine · 2024
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  11. [All-trans retinoic acid and vascular endothelial growth factor induced the directional osteogenic differentiation of mouse embryonic fibroblasts].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020
    Article
  12. Article
  13. Bone morphogenetic proteins 2, 6, and 9 differentially regulate the osteogenic differentiation of immortalized preodontoblasts.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2020
    Article
  14. Article
  15. Review
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  18. BMP9-induced osteoblastic differentiation requires functional Notch signaling in mesenchymal stem cells.Laboratory investigation; a journal of technical methods and pathology · 2019
    Article
  19. Article
  20. Article

9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors at 6 institutions in 2 countries.

Wenli ZhangDepartment of Orthopaedic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Zhong-Liang Deng
Liang Chen
Guo-Wei Zuo
Qing Luo
Qiong Shi
Bing-Qiang Zhang
Eric R Wagner
Farbod Rastegar
Stephanie H Kim
Wei Jiang
Jikun Shen
Enyi Huang
Yanhong Gao
Jian-Li Gao
Jian-Zhong Zhou
Jinyong Luo
Jiayi Huang
Xiaoji Luo
Yang Bi
Yuxi Su
Ke Yang
Hao Liu
Hue H Luu
Rex C Haydon
Tong-Chuan He
Bai-Cheng He
University of Chicago Medical Center · USChongqing Medical University · CNMolecular Oncology (United States) · USSichuan University · CNChongqing University · CNXinHua Hospital · CN

Funding

Center for Herbal Research on Colorectal CancerP01AT004418 · NCCIH · UNIVERSITY OF CHICAGO · PI YUAN, CHUN-SU · 2008 to 2012
$5.9M
Regulation of beta-Catenin Signaling by Tyrosine PhosphorylationR01CA106569 · NCI · UNIVERSITY OF CHICAGO · PI HE, TONG-CHUAN · 2006 to 2010
$1.3M
BMP6-Mediated OsteogenesisK08AR050142 · NIAMS · UNIVERSITY OF CHICAGO · PI HAYDON, REX C · 2004 to 2008
$596k
The role of connective Tissue Growth Factor on BMP9-Induced OsteogenesisK08AR054381 · NIAMS · UNIVERSITY OF CHICAGO · PI LUU, HUE HAN · 2007 to 2011
$595k
NCCIH NIH HHS AT004418NCCIH NIH HHS P01 AT004418NCI NIH HHS CA106569NCI NIH HHS R01 CA106569NIAMS NIH HHS AR054381NIAMS NIH HHS AR50142NIAMS NIH HHS K08 AR050142NIAMS NIH HHS K08 AR054381
6 · The paper itself

Abstract

backgroundAs one of the least studied bone morphogenetic proteins (BMPs), BMP9 is one of the most osteogenic BMPs. Retinoic acid (RA) signaling is known to play an important role in development, differentiation and bone metabolism. In this study, we investigate the effect of RA signaling on BMP9-induced osteogenic differentiation of mesenchymal progenitor cells (MPCs). METHODOLOGY/PRINCIPAL

findingsBoth primary MPCs and MPC line are used for BMP9 and RA stimulation. Recombinant adenoviruses are used to deliver BMP9, RARalpha and RXRalpha into MPCs. The in vitro osteogenic differentiation is monitored by determining the early and late osteogenic markers and matrix mineralization. Mouse perinatal limb explants and in vivo MPC implantation experiments are carried out to assess bone formation. We find that both 9CRA and ATRA effectively induce early osteogenic marker, such as alkaline phosphatase (ALP), and late osteogenic markers, such as osteopontin (OPN) and osteocalcin (OC). BMP9-induced osteogenic differentiation and mineralization is synergistically enhanced by 9CRA and ATRA in vitro. 9CRA and ATRA are shown to induce BMP9 expression and activate BMPR Smad-mediated transcription activity. Using mouse perinatal limb explants, we find that BMP9 and RAs act together to promote the expansion of hypertrophic chondrocyte zone at growth plate. Progenitor cell implantation studies reveal that co-expression of BMP9 and RXRalpha or RARalpha significantly increases trabecular bone and osteoid matrix formation. CONCLUSION/SIGNIFICANCE: Our results strongly suggest that retinoid signaling may synergize with BMP9 activity in promoting osteogenic differentiation of MPCs. This knowledge should expand our understanding about how BMP9 cross-talks with other signaling pathways. Furthermore, a combination of BMP9 and retinoic acid (or its agonists) may be explored as effective bone regeneration therapeutics to treat large segmental bony defects, non-union fracture, and/or osteoporotic fracture.

Indexed as

AnimalsBlotting, WesternCell DifferentiationCell LineCells, CulturedGrowth Differentiation Factor 2HumansMesenchymal Stem CellsMiceOrgan Culture TechniquesOsteogenesisReceptors, Retinoic AcidRetinoic Acid Receptor alphaReverse Transcriptase Polymerase Chain ReactionTretinoinGrowth Differentiation Factor 2RARA protein, humanRara protein, mouseReceptors, Retinoic AcidRetinoic Acid Receptor alphaTretinoin

Identifiers

PMID20689834
PMCPMC2912873
OpenAlexW2021239966

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.