ArticleThe Journal of biological chemistry2010
Uptake of selenite by Saccharomyces cerevisiae involves the high and low affinity orthophosphate transporters.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 86 citations in OpenAlex.
- Biogenic Selenium Nanoparticles from Food-GradeFoods (Basel, Switzerland) · 2026Article
- Comparative Study on the Effects of Selenium-Enriched Yeasts with Different Selenomethionine Contents on Gut Microbiota and Metabolites.International journal of molecular sciences · 2025Article
- Characterization and biological activity of selenium nanoparticles biosynthesized by Yarrowia lipolytica.Microbial biotechnology · 2024Article
- Transcriptome analysis of high- and low-selenium genotypes identifies genes responsible for selenium absorption, translocation, and accumulation.Frontiers in plant science · 2024Article
- Mechanistic characterization of waterborne selenite uptake in the water flea,Conservation physiology · 2024Article
- Optimization of Se- and Zn-Enriched Mycelium ofNutrients · 2023Article
- Adaptive Laboratory Evolution Reveals the Selenium Efflux Process To Improve Selenium Tolerance Mediated by the Membrane Sulfite Pump in Saccharomyces cerevisiae.Microbiology spectrum · 2023Article
- Article
- Selenium uptake, translocation, subcellular distribution and speciation in winter wheat in response to phosphorus application combined with three types of selenium fertilizer.BMC plant biology · 2023Article
- Comparative transcriptomics provides novel insights into the mechanisms of selenium accumulation and transportation in tea cultivars (Frontiers in plant science · 2023Article
- Review
- Selenium Metabolism and Selenoproteins in Prokaryotes: A Bioinformatics Perspective.Biomolecules · 2022Review
- A Similarity-Based Method for Predicting Enzymatic Functions in Yeast Uncovers a New AMP Hydrolase.Journal of molecular biology · 2022Article
- Molecular Biology of Cadmium Toxicity in Saccharomyces cerevisiae.Biological trace element research · 2021Review
- Mechanisms Affecting the Biosynthesis and Incorporation Rate of Selenocysteine.Molecules (Basel, Switzerland) · 2021Review
- Interactions of Silicon With Essential and Beneficial Elements in Plants.Frontiers in plant science · 2021Review
- Metabolic Response of the YeastInternational journal of molecular sciences · 2020Article
- Selenium Biofortification of Crop Food by Beneficial Microorganisms.Journal of fungi (Basel, Switzerland) · 2020Review
- Phosphate Homeostasis - A Vital Metabolic Equilibrium Maintained Through the INPHORS Signaling Pathway.Frontiers in microbiology · 2020Review
- Improvement of selenium enrichment inBioengineered · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the general cytotoxicity of selenite is well established, the mechanism by which this compound crosses cellular membranes is still unknown. Here, we show that in Saccharomyces cerevisiae, the transport system used opportunistically by selenite depends on the phosphate concentration in the growth medium. Both the high and low affinity phosphate transporters are involved in selenite uptake. When cells are grown at low P(i) concentrations, the high affinity phosphate transporter Pho84p is the major contributor to selenite uptake. When phosphate is abundant, selenite is internalized through the low affinity P(i) transporters (Pho87p, Pho90p, and Pho91p). Accordingly, inactivation of the high affinity phosphate transporter Pho84p results in increased resistance to selenite and reduced uptake in low P(i) medium, whereas deletion of SPL2, a negative regulator of low affinity phosphate uptake, results in exacerbated sensitivity to selenite. Measurements of the kinetic parameters for selenite and phosphate uptake demonstrate that there is a competition between phosphate and selenite ions for both P(i) transport systems. In addition, our results indicate that Pho84p is very selective for phosphate as compared with selenite, whereas the low affinity transporters discriminate less efficiently between the two ions. The properties of phosphate and selenite transport enable us to propose an explanation to the paradoxical increase of selenite toxicity when phosphate concentration in the growth medium is raised above 1 mm.
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