ArticleThe Journal of molecular diagnostics : JMD2010
Diagnostic testing for pandemic influenza in Singapore: a novel dual-gene quantitative real-time RT-PCR for the detection of influenza A/H1N1/2009.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Detection of infectious agents in lungs of slaughtered pigs in association with cranioventral pulmonary consolidation.Journal of veterinary research · 2025Article
- Infectivity and transmissibility of an avian H3N1 influenza virus in pigs.Veterinary research · 2023Article
- Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settings.Scientific reports · 2022Article
- Year‑Round Influenza A Virus Surveillance in Mallards (Viruses · 2020Article
- Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2.Theranostics · 2020Review
- Bioaerosol Sampling for Respiratory Viruses in Singapore's Mass Rapid Transit Network.Scientific reports · 2018Article
- Mutations in the Influenza A Virus M1 Protein Enhance Virus Budding To Complement Lethal Mutations in the M2 Cytoplasmic Tail.Journal of virology · 2018Article
- Contamination-controlled high-throughput whole genome sequencing for influenza A viruses using the MiSeq sequencer.Scientific reports · 2016Article
- Evaluation of the immunogenicity and protective effects of a trivalent chimeric norovirus P particle immunogen displaying influenza HA2 from subtypes H1, H3 and B.Emerging microbes & infections · 2016Article
- Molecular surveillance of antiviral drug resistance of influenza A/H3N2 virus in Singapore, 2009-2013.PloS one · 2015Article
- Emergence of G186D mutation in the presence of R292K mutation in an immunocompromised child infected with influenza A/H3N2 virus, treated with oseltamivir.Journal of clinical microbiology · 2014Article
- Development of one-step real-time PCR assay for titrating trivalent live attenuated influenza vaccines.Human vaccines & immunotherapeutics · 2014Article
- Modular glycosphere assays for high-throughput functional characterization of influenza viruses.BMC biotechnology · 2013Article
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- microRNAs in circulation are altered in response to influenza A virus infection in humans.PloS one · 2013Article
- Article
- Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City, Vietnam.Diagnostic microbiology and infectious disease · 2012Article
- A universal influenza A and B duplex real-time RT-PCR assay.Journal of medical virology · 2012Article
- High-resolution melting approach to efficient identification and quantification of H275Y mutant influenza H1N1/2009 virus in mixed-virus-population samples.Journal of clinical microbiology · 2011Article
- Comparison of pandemic (H1N1) 2009 and seasonal influenza viral loads, Singapore.Emerging infectious diseases · 2011Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the relative global lack of immunity to the pandemic influenza A/H1N1/2009 virus that emerged in April 2009 as well as the sustained susceptibility to infection, rapid and accurate diagnostic assays are essential to detect this novel influenza A variant. Among the molecular diagnostic methods that have been developed to date, most are in tandem monoplex assays targeting either different regions of a single viral gene segment or different viral gene segments. We describe a dual-gene (duplex) quantitative real-time RT-PCR method selectively targeting pandemic influenza A/H1N1/2009. The assay design includes a primer-probe set specific to only the hemagglutinin (HA) gene of this novel influenza A variant and a second set capable of detecting the nucleoprotein (NP) gene of all swine-origin influenza A virus. In silico analysis of the specific HA oligonucleotide sequence used in the assay showed that it targeted only the swine-origin pandemic strain; there was also no cross-reactivity against a wide spectrum of noninfluenza respiratory viruses. The assay has a diagnostic sensitivity and specificity of 97.7% and 100%, respectively, a lower detection limit of 50 viral gene copies/PCR, and can be adapted to either a qualitative or quantitative mode. It was first applied to 3512 patients with influenza-like illnesses at a tertiary hospital in Singapore, during the containment phase of the pandemic (May to July 2009).
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