ArticleAnnals of biomedical engineering2011
Simultaneous measurement of anisotropic solute diffusivity and binding reaction rates in biological tissues by FRAP.
Article in Annals of biomedical engineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Human mesenchymal stem/stromal cell-derived extracellular vesicle transport in meniscus fibrocartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue.PloS one · 2024Article
- Strain-Dependent Diffusivity of Small and Large Molecules in Meniscus.Journal of biomechanical engineering · 2022Article
- A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion.Nature communications · 2021Article
- EFFECTS OF SOLUTE SIZE AND TISSUE COMPOSITION ON MOLECULAR AND MACROMOLECULAR DIFFUSIVITY IN HUMAN KNEE CARTILAGE.Osteoarthritis and cartilage open · 2020Article
- Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.Osteoarthritis and cartilage · 2020Article
- Molecular transport in articular cartilage - what have we learned from the past 50 years?Nature reviews. Rheumatology · 2018Review
- Enzyme-Induced Matrix Softening Regulates Hepatocarcinoma Cancer Cell Phenotypes.Macromolecular bioscience · 2017Article
- Conformational dynamics accompanying the proteolytic degradation of trimeric collagen I by collagenases.Journal of the American Chemical Society · 2012Article
- Strain tunes proteolytic degradation and diffusive transport in fibrin networks.Biomacromolecules · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Several solutes (e.g., growth factors, cationic solutes, etc.) can reversibly bind to the extracellular matrix (ECM) of biological tissues. Binding interactions have significant implications on transport of such solutes through the ECM. In order to fully delineate transport phenomena in biological tissues, knowledge of binding kinetics is crucial. In this study, a new method for the simultaneous determination of solute anisotropic diffusivity and binding reaction rates was presented. The new technique was solely based on Fourier analysis of fluorescence recovery after photobleaching (FRAP) images. Computer-simulated FRAP tests were used to assess the sensitivity and the robustness of the method to experimental parameters, such as anisotropic solute diffusivity and rates of binding reaction. The new method was applied to the determination of diffusivity and binding rates of 5-dodecanoylaminofluorescein (DAF) in bovine coccygeal annulus fibrosus (AF). Our findings indicate that DAF reversibly binds to the ECM of AF. In addition, it was found that DAF diffusion in AF is anisotropic. The results were in agreement with those reported in previous studies. This study provides a new tool for the simultaneous determination of solute anisotropic diffusion tensor and rates of binding reaction that can be used to investigate diffusive-reactive transport in biological tissues and tissue engineered constructs.
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