ArticlePloS one2010
Screening of in vivo activated genes in Enterococcus faecalis during insect and mouse infections and growth in urine.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
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- Identification ofMicroorganisms · 2021Article
- Application of Recombinase-Based In Vivo Expression Technology toMicroorganisms · 2020Article
- Adaptation to Adversity: the Intermingling of Stress Tolerance and Pathogenesis in Enterococci.Microbiology and molecular biology reviews : MMBR · 2019Review
- A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection.Scientific reports · 2018Article
- Review
- In vivo expression technology and 5' end mapping of the Borrelia burgdorferi transcriptome identify novel RNAs expressed during mammalian infection.Nucleic acids research · 2017Article
- Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.Virulence · 2016Review
- Killing of VRE Enterococcus faecalis by commensal strains: Evidence for evolution and accumulation of mobile elements in the absence of competition.Gut microbes · 2016Article
- Pheromone killing of multidrug-resistant Enterococcus faecalis V583 by native commensal strains.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Proteomic Investigation of the Response of Enterococcus faecalis V583 when Cultivated in Urine.PloS one · 2015Article
- Enterococcus faecalis 6-phosphogluconolactonase is required for both commensal and pathogenic interactions with Manduca sexta.Infection and immunity · 2015Article
- The Intraperitoneal Transcriptome of the Opportunistic Pathogen Enterococcus faecalis in Mice.PloS one · 2015Article
- Genomic comparative analysis of the environmental Enterococcus mundtii against enterococcal representative species.BMC genomics · 2014Article
- Article
- Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease.World journal of gastroenterology · 2013Article
- In vivo gene expression of Pseudomonas putida KT2440 in the rhizosphere of different plants.Microbial biotechnology · 2013Article
- In vivo expression technology identifies a novel virulence factor critical for Borrelia burgdorferi persistence in mice.PLoS pathogens · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococcus faecalis is part of the commensal microbiota of humans and its main habitat is the gastrointestinal tract. Although harmless in healthy individuals, E. faecalis has emerged as a major cause of nosocomial infections. In order to better understand the transformation of a harmless commensal into a life-threatening pathogen, we developed a Recombination-based In VivoExpression Technology for E. faecalis. Two R-IVET systems with different levels of sensitivity have been constructed in a E. faecalis V583 derivative strain and tested in the insect model Galleria mellonella, during growth in urine, in a mouse bacteremia and in a mouse peritonitis model. Our combined results led to the identification of 81 in vivo activated genes. Among them, the ef_3196/7 operon was shown to be strongly induced in the insect host model. Deletion of this operonic structure demonstrated that this two-component system was essential to the E. faecalis pathogenic potential in Galleria. Gene ef_0377, induced in insect and mammalian models, has also been further analyzed and it has been demonstrated that this ankyrin-encoding gene was also involved in E. faecalis virulence. Thus these R-IVET screenings led to the identification of new E. faecalis factors implied in in vivo persistence and pathogenic potential of this opportunistic pathogen.
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