ArticleNature2010
Branched tricarboxylic acid metabolism in Plasmodium falciparum.
Article in Nature, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 120 citations in OpenAlex.
- Targeting Malaria's Achilles' Heels: A Review of Plasmodium Life Cycle Vulnerabilities for Drug Discovery.Current topics in medicinal chemistry · 2025Review
- Cyto-Genotoxic and Behavioral Effects of Flubendiamide inInternational journal of molecular sciences · 2023Article
- Extraction and determination of flubendiamide insecticide in food samples: A review.Current research in food science · 2022Review
- Redox interactome in malaria parasite Plasmodium falciparum.Parasitology research · 2021Review
- Novel chloroquine loaded curcumin based anionic linear globular dendrimer G2: a metabolomics study onParasitology · 2020Article
- Analysis of the active fraction of IranianIranian journal of basic medical sciences · 2020Article
- Identification of Novel Plasmodium falciparum Hexokinase Inhibitors with Antiparasitic Activity.Antimicrobial agents and chemotherapy · 2016Article
- Stable isotope-labeled tracers for metabolic pathway elucidation by GC-MS and FT-MS.Methods in molecular biology (Clifton, N.J.) · 2014Article
- The use of functional genomics in conjunction with metabolomics for Mycobacterium tuberculosis research.Disease markers · 2014Review
- Phosphoenolpyruvate carboxylase identified as a key enzyme in erythrocytic Plasmodium falciparum carbon metabolism.PLoS pathogens · 2014Article
- Systems biological approaches to measure and understand vaccine immunity in humans.Seminars in immunology · 2013Review
- A key role for lipoic acid synthesis during Plasmodium liver stage development.Cellular microbiology · 2013Article
- Interrogating a hexokinase-selected small-molecule library for inhibitors of Plasmodium falciparum hexokinase.Antimicrobial agents and chemotherapy · 2013Article
- The metabolic roles of the endosymbiotic organelles of Toxoplasma and Plasmodium spp.Current opinion in microbiology · 2013Review
- Mitochondrial metabolism of sexual and asexual blood stages of the malaria parasite Plasmodium falciparum.BMC biology · 2013Article
- Parallel labeling experiments and metabolic flux analysis: Past, present and future methodologies.Metabolic engineering · 2013Review
- Impaired skeletal muscle microvascular function and increased skeletal muscle oxygen consumption in severe falciparum malaria.The Journal of infectious diseases · 2013Article
- Effects of Neospora caninum infection on brain microvascular endothelial cells bioenergetics.Parasites & vectors · 2013Article
- A host GPCR signaling network required for the cytolysis of infected cells facilitates release of apicomplexan parasites.Cell host & microbe · 2013Article
- Extraction of hydrophilic metabolites from Plasmodium falciparum-infected erythrocytes for metabolomic analysis.Methods in molecular biology (Clifton, N.J.) · 2013Article
Corrections and comments
- Correction · 2011-01-19Error in Data · Updated to Retraction ·
- Retraction · 2013-04-24Error in Methods · Error in Results and/or Conclusions · Unreliable Results and/or Conclusions · Upgrade/Update of Prior Notice(s) ·
- Commented on by
- Erratum issued
- Retracted
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A central hub of carbon metabolism is the tricarboxylic acid cycle, which serves to connect the processes of glycolysis, gluconeogenesis, respiration, amino acid synthesis and other biosynthetic pathways. The protozoan intracellular malaria parasites (Plasmodium spp.), however, have long been suspected of possessing a significantly streamlined carbon metabolic network in which tricarboxylic acid metabolism plays a minor role. Blood-stage Plasmodium parasites rely almost entirely on glucose fermentation for energy and consume minimal amounts of oxygen, yet the parasite genome encodes all of the enzymes necessary for a complete tricarboxylic acid cycle. Here, by tracing (13)C-labelled compounds using mass spectrometry we show that tricarboxylic acid metabolism in the human malaria parasite Plasmodium falciparum is largely disconnected from glycolysis and is organized along a fundamentally different architecture from the canonical textbook pathway. We find that this pathway is not cyclic, but rather is a branched structure in which the major carbon sources are the amino acids glutamate and glutamine. As a consequence of this branched architecture, several reactions must run in the reverse of the standard direction, thereby generating two-carbon units in the form of acetyl-coenzyme A. We further show that glutamine-derived acetyl-coenzyme A is used for histone acetylation, whereas glucose-derived acetyl-coenzyme A is used to acetylate amino sugars. Thus, the parasite has evolved two independent production mechanisms for acetyl-coenzyme A with different biological functions. These results significantly clarify our understanding of the Plasmodium metabolic network and highlight the ability of altered variants of central carbon metabolism to arise in response to unique environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.