ArticleThe ISME journal2011
Salmonella transcriptional signature in Tetrahymena phagosomes and role of acid tolerance in passage through the protist.
Article in The ISME journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- The Phase-Specific Dynamics in Gene Expression of Salmonella Typhimurium During Acanthamoeba castellanii Infection.Current microbiology · 2025Article
- Tetrahymena predation drives adaptive evolution of Salmonella by disrupting O-antigen biosynthesis and upregulating transcriptional regulator csgD.The ISME journal · 2025Article
- Making a Pathogen? Evaluating the Impact of Protist Predation on the Evolution of Virulence in Serratia marcescens.Genome biology and evolution · 2024Article
- Comparative transcriptome combined with morphophysiological analyses revealed the molecular mechanism underlyingVirulence · 2022Article
- Microbial warfare in the wild-the impact of protists on the evolution and virulence of bacterial pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence forInternational journal of molecular sciences · 2021Article
- Vibrio cholerae residing in food vacuoles expelled by protozoa are more infectious in vivo.Nature microbiology · 2019Article
- Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomics.Microbiome · 2019Article
- Diverse effects of nitric oxide reductase NorV on Aeromonas hydrophila virulence-associated traits under aerobic and anaerobic conditions.Veterinary research · 2019Article
- Article
- Relevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum.Frontiers in microbiology · 2016Article
- Identification ofFrontiers in cellular and infection microbiology · 2016Article
- Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria.Frontiers in microbiology · 2014Article
- The salmonella transcriptome in lettuce and cilantro soft rot reveals a niche overlap with the animal host intestine.Applied and environmental microbiology · 2013Article
- Article
- Distinct transcriptional profiles and phenotypes exhibited by Escherichia coli O157:H7 isolates related to the 2006 spinach-associated outbreak.Applied and environmental microbiology · 2012Article
- Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.Methods in cell biology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica Typhimurium remains undigested in the food vacuoles of the common protist, Tetrahymena. Contrary to its interaction with Acanthamoeba spp., S. Typhimurium is not cytotoxic to Tetrahymena and is egested as viable cells in its fecal pellets. Through microarray gene expression profiling we investigated the factors in S. Typhimurium that are involved in its resistance to digestion by Tetrahymena. The transcriptome of S. Typhimurium in Tetrahymena phagosomes showed that 989 and 1282 genes were altered in expression compared with that in water and in LB culture medium, respectively. A great proportion of the upregulated genes have a role in anaerobic metabolism and the use of alternate electron acceptors. Many genes required for survival and replication within macrophages and human epithelial cells also had increased expression in Tetrahymena, including mgtC, one of the most highly induced genes in all three cells types. A ΔmgtC mutant of S. Typhimurium did not show decreased viability in Tetrahymena, but paradoxically, was egested at a higher cell density than the wild type. The expression of adiA and adiY, which are involved in arginine-dependent acid resistance, also was increased in the protozoan phagosome. A ΔadiAY mutant had lower viability after passage through Tetrahymena, and a higher proportion of S. Typhimurium wild-type cells within pellets remained viable after exposure to pH 3.4 as compared with uningested cells. Our results provide evidence that acid resistance has a role in the resistance of Salmonella to digestion by Tetrahymena and that passage through the protist confers physiological advantages relevant to its contamination cycle.
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Registered trials
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