ArticleAmerican journal of physiology. Cell physiology2010
Characterization of hTERT-immortalized osteoblast cell lines generated from wild-type and connexin43-null mouse calvaria.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- Biofunctionalized zinc nanoparticles from Alangium salvifolium seeds extract: a potent antibacterial and anti-Cancer therapeutic agent.Journal, genetic engineering & biotechnology · 2026Article
- Panx1 and P2X7R are associated with impaired skeletal health and delayed bone development in early onset of type 1 diabetes.Frontiers in endocrinology · 2026Article
- Cell Immortality:Life (Basel, Switzerland) · 2024Review
- Connexin 43 hemichannels and prostaglandin EFrontiers in cell and developmental biology · 2023Review
- Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity.Nanomaterials (Basel, Switzerland) · 2021Article
- Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice.Frontiers in cellular neuroscience · 2021Article
- Connexins in Cancer: Jekyll or Hyde?Biomolecules · 2020Review
- Role of pannexin 1 channels in load-induced skeletal response.Annals of the New York Academy of Sciences · 2019Article
- Osteocyte calcium signals encode strain magnitude and loading frequency in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Connexins and Pannexins in Bone and Skeletal Muscle.Current osteoporosis reports · 2017Review
- Connexin43 and Runx2 Interact to Affect Cortical Bone Geometry, Skeletal Development, and Osteoblast and Osteoclast Function.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Article
- Inhibition of connexin 43 prevents trauma-induced heterotopic ossification.Scientific reports · 2016Article
- P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes.PloS one · 2016Article
- Article
- Adipocytes in both brown and white adipose tissue of adult mice are functionally connected via gap junctions: implications for Chagas disease.Microbes and infection · 2014Article
- Connexins modulate autophagosome biogenesis.Nature cell biology · 2014Article
- High bone mass in mice lacking Cx37 because of defective osteoclast differentiation.The Journal of biological chemistry · 2014Article
- Article
- Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue.Stem cells and development · 2013Article
- Matrix-dependent adhesion mediates network responses to physiological stimulation of the osteocyte cell process.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The gap junction protein connexin43 (Cx43) has been proposed to play key roles in bone differentiation and mineralization, but underlying cellular mechanisms are not totally understood. To further explore roles of Cx43 in these processes, we immortalized calvarial osteoblasts from wild-type and Cx43-null mice using human telomerase reverse transcriptase (hTERT). Osteoblastic (MOB) cell lines were generated from three individual wild-type and three individual Cx43-null mouse calvaria. Average population doubling times of the cell lines were higher than of the primary osteoblasts but did not greatly differ with regard to genotype. Modest to high level of Cx45 expression was detected in MOBs of both genotypes. Most of the cell lines expressed osteoblastic markers [Type I collagen, osteopontin, osteocalcin, parathyroid hormone/parathyroid hormone-related peptide receptor (PTH/PTHrP), periostin (OSF-2), osterix (Osx), runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP)], and mineralization was comparable to that of primary osteoblasts. Two MOB cell lines from each genotype with most robust maintenance of osteoblast lineage markers were analyzed in greater detail, revealing that the Cx43-null cell lines showed a significant delay in early differentiation (up to 9 days in culture). Matrix mineralization was markedly delayed in one of the Cx43-null lines and slightly delayed in the other. These findings comparing new and very stable wild-type and Cx43-null osteoblastic cell lines define a role for Cx43 in early differentiation and mineralization stages of osteoblasts and further support the concept that Cx43 plays important role in the cellular processes associated with skeleton function.
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