ArticleJournal of virology2010
A gC1qR prevents white spot syndrome virus replication in the freshwater crayfish Pacifastacus leniusculus.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 36 citations in OpenAlex.
- Role of Cellular Receptors in the Innate Immune System of Crustaceans in Response to White Spot Syndrome Virus.Viruses · 2022Review
- Identification of the gC1qR sites for the HIV-1 viral envelope protein gp41 and the HCV core protein: Implications in viral-specific pathogenesis and therapy.Molecular immunology · 2016Article
- Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments.Viruses · 2016Review
- Hijacking of host calreticulin is required for the white spot syndrome virus replication cycle.Journal of virology · 2014Article
- Calreticulin contributes to C1q-dependent recruitment of microglia in the leech Hirudo medicinalis following a CNS injury.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- Recent advances in crayfish hematopoietic stem cell culture: a model for studies of hemocyte differentiation and immunity.Cytotechnology · 2013Article
- Primary culture of hemocytes from Eriocheir sinensis and their immune effects to the novel crustacean pathogen Spiroplasma eriocheiris.Molecular biology reports · 2012Article
- Bacteria-Induced Dscam Isoforms of the Crustacean, Pacifastacus leniusculus.PLoS pathogens · 2011Article
Corrections and comments
- Retraction · 2015-12-20Author Unresponsive · Investigation by Company/Institution · Investigation by Journal/Publisher · Investigation by Third Party · Manipulation of Images ·
- Retracted
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gC1qR/p32 protein is a multiple receptor for several proteins and pathogens. We cloned a gC1qR homologue in a crustacean, Pacifastacus leniusculus, and analyzed the expression of P. leniusculus C1qR (PlgC1qR) in various tissues. The gC1qR/p32 transcript was significantly enhanced by white spot syndrome virus (WSSV) infection 6 h after viral infection both in vitro in a hematopoietic tissue cell culture (Hpt) and in vivo compared to appropriate controls. Moreover, PlgC1qR silencing in both the Hpt cell culture and live crayfish enhanced the WSSV replication. In addition, by making a recombinant PlgC1qR protein we could show that if this recombinant protein was injected in a crayfish, Pacifastacus leniusculus, followed by injection of WSSV, this significantly reduced viral replication in vivo. Furthermore, if the recombinant PlgC1qR was incubated with Hpt cells and then WSSV was added, this also reduced viral replication. These experiments clearly demonstrate that recombinant PlgC1qR reduce WSSV replication both in vivo and in vitro. The results from a far-Western overlay and glutathione S-transferase pull-down assays showed that PlgC1qR could bind to VP15, VP26, and VP28. Altogether, these results demonstrate a role for PlgC1qR in antiviral activity against WSSV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.