ArticleAnnals of clinical microbiology and antimicrobials2010
Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR.
Article in Annals of clinical microbiology and antimicrobials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Molecular study of specific virulence genes of Pseudomonas aeruginosa isolated from sub clinical bovine mastitis cases.Scientific reports · 2026Article
- Understanding the Burden and Management of Urinary Tract Infections in Women.Diseases (Basel, Switzerland) · 2025Review
- Hyper-Responsive Chemiluminescent Probe Reveals Distinct PYRase Activity inBioconjugate chemistry · 2024Article
- Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Appropriateness of empiric antimicrobial therapy with imipenem/colistin in severe septic patients: observational cohort study.Annals of clinical microbiology and antimicrobials · 2018Observational
- Recent Advances in Molecular Diagnosis ofFrontiers in microbiology · 2018Article
- An Improved In-house MALDI-TOF MS Protocol for Direct Cost-Effective Identification of Pathogens from Blood Cultures.Frontiers in microbiology · 2017Article
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- Highly sensitive and rapid detection of Pseudomonas aeruginosa based on magnetic enrichment and magnetic separation.Theranostics · 2013Article
- Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles.Journal of clinical microbiology · 2012Article
- Detection of bacterial 16S rRNA and identification of four clinically important bacteria by real-time PCR.PloS one · 2012Article
- Validation of PCR for the detection of Pseudomonas aeruginosa from corneal samples.International journal of ophthalmology · 2011Article
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Authors and funding
6 authors.
Funding
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Abstract
backgroundPseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification of P. aeruginosa from positive blood cultures (BCs).
methodsOver the period August 2008 to June 2009, 100 BC bottles positive for gram-negative bacilli were tested in order to evaluate performances of the qPCR technique with conventional methods as gold standard (i.e. culture and phenotypic identification).
resultsThirty-three strains of P. aeruginosa, 53 strains of Enterobactericaeae, nine strains of Stenotrophomonas maltophilia and two other gram-negative species were isolated while 3 BCs were polymicrobial including one mixture containing P. aeruginosa. All P. aeruginosa clinical isolates were detected by qPCR except a single strain in mixed culture. Performances of the qPCR technique were: specificity, 100%; positive predictive value, 100%; negative predictive value, 98.5%; and sensitivity, 97%.
conclusionsThis reliable technique may offer a rapid (<1.5 h) tool that would help clinicians to initiate an appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of this novel strategy as compared to phenotypic methods.
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