ArticleTissue engineering. Part C, Methods2011
Tissue-engineered model for real-time monitoring of liver inflammation.
Article in Tissue engineering. Part C, Methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 12 citations in OpenAlex.
- A Cell Culture Platform to Maintain Long-term Phenotype of Primary Human Hepatocytes and Endothelial Cells.Cellular and molecular gastroenterology and hepatology · 2018Article
- Emerging In Vitro Liver Technologies for Drug Metabolism and Inter-Organ Interactions.Tissue engineering. Part B, Reviews · 2016Review
- A Novel Resolvin-Based Strategy for Limiting Acetaminophen Hepatotoxicity.Clinical and translational gastroenterology · 2016Article
- Long-term coculture strategies for primary hepatocytes and liver sinusoidal endothelial cells.Tissue engineering. Part C, Methods · 2015Article
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
- New Methods in Tissue Engineering: Improved Models for Viral Infection.Annual review of virology · 2014Article
- Dynamic interplay of flow and collagen stabilizes primary hepatocytes culture in a microfluidic platform.Lab on a chip · 2014Article
- High throughput micro-well generation of hepatocyte micro-aggregates for tissue engineering.PloS one · 2014Article
- Article
- The inflammatory response to double stranded DNA in endothelial cells is mediated by NFκB and TNFα.PloS one · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Tissue-engineered in vitro models have the potential to be used for investigating inflammation in the complex microenvironment found in vivo. We have developed an in vitro model of hepatic tissue that facilitates real-time monitoring of endothelium activation in liver tissue. This was achieved by creating a layered coculture model in which hepatocytes were embedded in collagen gel and a reporter clone of endothelial cells, which synthesizes green fluorescent protein in response to nuclear factor-kappa B (NF-κB) activation, was overlaid on top of the gel. The efficacy of our approach was established by monitoring in real time the dynamics of NF-κB-regulated fluorescence in response to tumor necrosis factor α. Our studies revealed that endothelial cells in coculture with hepatocytes exhibited a similar NF-κB-mediated fluorescence to both pulse and step stimulation of lipopolysaccharide. By contrast, endothelial cells in monoculture displayed enhanced NF-κB-regulated fluorescence to step in comparison to pulse lipopolysaccharide stimulation. The NF-κB-mediated fluorescence correlated with endothelial cell expression of NF-κB-regulated genes such as intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and E-Selectin, as well as with leukocyte adhesion. These findings suggest that our model provides a powerful platform for investigating hepatic endothelium activation in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.