ArticleCellular & molecular biology letters2010
Leptin regulates MMP-2, TIMP-1 and collagen synthesis via p38 MAPK in HL-1 murine cardiomyocytes.
Article in Cellular & molecular biology letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Engineering an immune-integrated lung-on-a-chip to reveal TOX-RAGE axis-driven fibrosis and RAGE blockade as a therapeutic strategy.Nano convergence · 2025Article
- Leptin as a key driver for organ fibrogenesis.Science advances · 2025Article
- β-Nicotinamide mononucleotide blocks UVB-induced collagen reduction via regulation of ROS/MAPK/AP-1 and stimulation of mitochondrial proline biosynthesis.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Molecular Pathways and Animal Models of Cardiomyopathies.Advances in experimental medicine and biology · 2024Article
- The emerging role of leptin in obesity-associated cardiac fibrosis: evidence and mechanism.Molecular and cellular biochemistry · 2023Review
- Genetic inhibition of serum glucocorticoid kinase 1 prevents obesity-related atrial fibrillation.JCI insight · 2022Article
- Remodeling and Fibrosis of the Cardiac Muscle in the Course of Obesity-Pathogenesis and Involvement of the Extracellular Matrix.International journal of molecular sciences · 2022Review
- Analysis of myocardial cellular gene expression during pressure overload reveals matrix based functional intercellular communication.iScience · 2022Article
- Decreased Collagen Type I is Associated with Increased Metalloproteinase-2 Activity and Protein Expression of Leptin in the Myocardium of Obese Rats.Arquivos brasileiros de cardiologia · 2020Article
- Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heartJCI insight · 2019Article
- Leptin and Immunological Profile in Obesity and Its Associated Diseases in Dogs.International journal of molecular sciences · 2019Review
- Effects of AT1 receptor antagonism on interstitial and ultrastructural remodeling of heart in response to a hypercaloric diet.Physiological reports · 2019Article
- Cleavage of the leptin receptor by matrix metalloproteinase-2 promotes leptin resistance and obesity in mice.Science translational medicine · 2018Article
- MicroRNA-93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells.Cell proliferation · 2015Article
- Progesterone Inhibits Leptin-Induced Invasiveness of BeWo Cells.International journal of medical sciences · 2015Article
- Inhibition of leptin-induced vascular extracellular matrix remodelling by adiponectin.Journal of molecular endocrinology · 2014Article
- Exogenous hydrogen sulfide alleviates high glucose-induced cardiotoxicity via inhibition of leptin signaling in H9c2 cells.Molecular and cellular biochemistry · 2014Article
- Influence of term of exposure to high-fat diet-induced obesity on myocardial collagen type I and III.Arquivos brasileiros de cardiologia · 2014Article
- Lifestyle Modification with Diet and Exercise in Obese Patients with Heart Failure - A Pilot Study.Journal of obesity & weight loss therapy · 2012Article
- A resealed-cell system for analyzing pathogenic intracellular events: perturbation of endocytic pathways under diabetic conditions.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A clear association between obesity and heart failure exists and a significant role for leptin, the product of the obese gene, has been suggested. One aspect of myocardial remodeling which characterizes heart failure is a disruption in the balance of extracellular matrix synthesis and degradation. Here we investigated the effects of leptin on matrix metalloproteinase (MMP) activity, tissue inhibitor of metalloproteinase (TIMP) expression, as well as collagen synthesis in HL-1 cardiac muscle cells. Gelatin zymographic analysis of MMP activity in conditioned media showed that leptin enhanced MMP-2 activity in a dose- and time-dependent manner. Leptin is known to stimulate phosphorylation of p38 MAPK in cardiac cells and utilization of the p38 MAPK inhibitor, SB203580, demonstrated that this kinase also plays a role in regulating several extracellular matrix components, such that inhibition of p38 MAPK signaling prevented the leptin-induced increase in MMP-2 activation. We also observed that leptin enhanced collagen synthesis determined by both proline incorporation and picrosirius red staining of conditioned media. Pro-collagen type-I and pro-collagen type-III expression, measured by real-time PCR and Western blotting were also increased by leptin, effects which were again attenuated by SB203580. In summary, these results demonstrate the potential for leptin to play a role in mediating myocardial ECM remodeling and that the p38 MAPK pathway plays an important role in mediating these effects.
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