ArticleJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism2011
Metabolic acidosis induced by Plasmodium falciparum intraerythrocytic stages alters blood-brain barrier integrity.
Article in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 42 citations in OpenAlex.
- Review
- Blood pH Analysis in Combination with Molecular Medical Tools in Relation to COVID-19 Symptoms.Biomedicines · 2023Article
- Mg-based materials diminish tumor spreading and cancer metastases.Bioactive materials · 2023Article
- Cerebral malaria - modelling interactions at the blood-brain barrier in vitro.Disease models & mechanisms · 2022Review
- General Structural and Functional Features of Molecular Chaperones.Advances in experimental medicine and biology · 2021Article
- Cerebral Plasmodium falciparum malaria: The role of PfEMP1 in its pathogenesis and immunity, and PfEMP1-based vaccines to prevent it.Immunological reviews · 2020Review
- The Natural Compound Climacostol as a Prodrug Strategy Based on pH Activation for Efficient Delivery of Cytotoxic Small Agents.Frontiers in chemistry · 2019Article
- Comparative 1D Blue-Native electrophoresis analysis of Plasmodium falciparum and human proteins associated with cytoadherence.Malaria journal · 2018Article
- Development of a direct contact astrocyte-human cerebral microvessel endothelial cells blood-brain barrier coculture model.The Journal of pharmacy and pharmacology · 2017Article
- Comparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with Plasmodium falciparum field isolates.BMC infectious diseases · 2017Article
- Dynamic interactions of Plasmodium spp. with vascular endothelium.Tissue barriers · 2017Review
- Angiotensin receptors and β-catenin regulate brain endothelial integrity in malaria.The Journal of clinical investigation · 2016Article
- The transporter and permeability interactions of asymmetric dimethylarginine (ADMA) and L-arginine with the human blood-brain barrier in vitro.Brain research · 2016Article
- Plasmodium falciparum proteins involved in cytoadherence of infected erythrocytes to chemokine CX3CL1.Scientific reports · 2016Article
- Plasmodium falciparum antigenic variation: relationships between widespread endothelial activation, parasite PfEMP1 expression and severe malaria.BMC infectious diseases · 2014Article
- Imaging Plasmodium immunobiology in the liver, brain, and lung.Parasitology international · 2014Review
- Host matrix metalloproteinases in cerebral malaria: new kids on the block against blood-brain barrier integrity?Fluids and barriers of the CNS · 2014Article
- P. falciparum isolate-specific distinct patterns of induced apoptosis in pulmonary and brain endothelial cells.PloS one · 2014Article
- Contrasting pediatric and adult cerebral malaria: the role of the endothelial barrier.Virulence · 2013Review
- The hCMEC/D3 cell line as a model of the human blood brain barrier.Fluids and barriers of the CNS · 2013Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenesis of cerebral malaria (CM) remains largely unknown. There is growing evidence that combination of both parasite and host factors could be involved in blood-brain barrier (BBB) breakdown. However, lack of adequate in vitro model of human BBB so far hampered molecular studies. In this article, we propose the use of hCMEC/D3 cells, a well-established human cerebral microvascular endothelial cell (EC) line, to study BBB breakdown induced by Plasmodium falciparum-parasitized red blood cells and environmental conditions. We show that coculture of parasitized erythrocytes with hCMEC/D3 cells induces cell adhesion and paracellular permeability increase, which correlates with disorganization of zonula occludens protein 1 expression pattern. Permeability increase and modification of tight junction proteins distribution are cytoadhesion independent. Finally, we show that permeability of hCMEC/D3 cell monolayers is mediated through parasite induced metabolic acidosis, which in turns correlates with apoptosis of parasitized erythrocytes. This new coculture model represents a very useful tool, which will improve the knowledge of BBB breakdown and the development of adjuvant therapies, together with antiparasitic drugs.
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