ArticleDiabetes2010
Acute stimulation of white adipocyte respiration by PKA-induced lipolysis.
Article in Diabetes, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 113 citations in OpenAlex.
- Fatty acids promote uncoupled respiration via ATP/ADP carriers in white adipocytes.Nature metabolism · 2026Article
- Ultrastructural characterization of white adipocytes in a mouse model with enhanced sequestration of fatty acids in adipose tissue.Adipocyte · 2025Article
- Vitamin D receptor signalling regulates the diet-driven metabolic shift during weaning.Molecular metabolism · 2025Article
- Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes.Adipocyte · 2024Article
- Thermogenic Adipocytes Promote M2 Macrophage Polarization through CNNM4-Mediated Mg Secretion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Gene Regulation and Mitochondrial Activity During White and Brown Adipogenesis Are Modulated by KDM5 Histone Demethylase.Journal of the Endocrine Society · 2024Article
- Mitophagy Responds to the Environmental Temperature and Regulates Mitochondrial Mass in Adipose Tissues.Advances in experimental medicine and biology · 2024Review
- The pseudokinase TRIB3 controls adipocyte lipid homeostasis and proliferation in vitro and in vivo.Molecular metabolism · 2023Article
- Brown adipocyte and browning thermogenesis: metabolic crosstalk beyond mitochondrial limits and physiological impacts.Adipocyte · 2023Review
- Standardized In Vitro Models of Human Adipose Tissue Reveal Metabolic Flexibility in Brown Adipocyte Thermogenesis.Endocrinology · 2023Article
- Synergistic Effects of Heat-Treated Green Tea Extract and Enzymatically-Modified Isoquercitrin in Preventing Obesity.Nutrients · 2023Article
- Thermogenic adipocyte-derived zinc promotes sympathetic innervation in male mice.Nature metabolism · 2023Article
- Expression of Functional Cannabinoid Type-1 (CBCells · 2022Article
- Adipocyte lysoplasmalogenase TMEM86A regulates plasmalogen homeostasis and protein kinase A-dependent energy metabolism.Nature communications · 2022Article
- Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat.Molecular metabolism · 2022Article
- An enriched environment re-establishes metabolic homeostasis by reducing obesity-induced inflammation.Disease models & mechanisms · 2022Article
- Preventing White Adipocyte Browning during DifferentiationStem cells international · 2022Article
- Isothermal microcalorimetry measures UCP1-mediated thermogenesis in mature brite adipocytes.Communications biology · 2021Article
- Integrated metabolomics and transcriptomics reveal the anti-aging effect of melanin fromAging · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWe examined the effect of β-adrenergic receptor (βAR) activation and cAMP-elevating agents on respiration and mitochondrial uncoupling in human adipocytes and probed the underlying molecular mechanisms. RESEARCH DESIGN AND
methodsOxygen consumption rate (OCR, aerobic respiration) and extracellular acidification rate (ECAR, anaerobic respiration) were examined in response to isoproterenol (ISO), forskolin (FSK), and dibutyryl-cAMP (DB), coupled with measurements of mitochondrial depolarization, lipolysis, kinase activities, and gene targeting or knock-down approaches.
resultsISO, FSK, or DB rapidly increased oxidative and glycolytic respiration together with mitochondrial depolarization in human and mouse white adipocytes. The increase in OCR was oligomycin-insensitive and contingent on cAMP-dependent protein kinase A (PKA)-induced lipolysis. This increased respiration and the uncoupling were blocked by inhibiting the mitochondrial permeability transition pore (PTP) and its regulator, BAX. Interestingly, compared with lean individuals, adipocytes from obese subjects exhibited reduced OCR and uncoupling capacity in response to ISO.
conclusionsLipolysis stimulated by βAR activation or other maneuvers that increase cAMP levels in white adipocytes acutely induces mitochondrial uncoupling and cellular energetics, which are amplified in the absence of scavenging BSA. The increase in OCR is dependent on PKA-induced lipolysis and is mediated by the PTP and BAX. Because this effect is reduced with obesity, further exploration of this uncoupling mechanism will be needed to determine its cause and consequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.