ArticleBiophysical journal2010
Microimaging of oxygen concentration near live photosynthetic cells by electron spin resonance.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Celebration of 80 years of EPR.Applied magnetic resonance · 2024Article
- Rapid Scan EPR Oxygen Imaging in Photoactivated Resin Used for Stereolithographic 3D Printing.3D printing and additive manufacturing · 2021Article
- Triarylmethyl Radicals: EPR Study ofZeitschrift fur physikalische Chemie (Frankfurt am Main, Germany) · 2017Article
- Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo.Journal of visualized experiments : JoVE · 2016Article
- Facile and High-Yielding Synthesis of TAM Biradicals and Monofunctional TAM Radicals.Synlett : accounts and rapid communications in synthetic organic chemistry · 2016Article
- Preparation of Diversely Substituted Triarylmethyl Radicals by the Quenching of Tris(2,3,5,6-tetrathiaaryl)methyl Cations with C-, N-, P-, and S-Nucleophiles.European journal of organic chemistry · 2014Article
- Hyperfine interactions of narrow-line trityl radical with solvent molecules.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2013Article
- Generation of Trityl Radicals by Nucleophilic Quenching of Tris(2,3,5,6-tetrathiaaryl)methyl Cations and Practical and Convenient Large-Scale Synthesis of Persistent Tris(4-carboxy-2,3,5,6-tetrathiaaryl)methyl Radical.European journal of organic chemistry · 2013Article
- Triarylmethanols bearing bulky aryl groups and the NOESY/EXSY experimental observation of two-ring-flip mechanism for helicity reversal of molecular propellers.European journal of organic chemistry · 2012Article
- Electron spin resonance micro-imaging of live species for oxygen mapping.Journal of visualized experiments : JoVE · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We present what is, to our knowledge, a new methodology for high-resolution three-dimensional imaging of oxygen concentration near live cells. The cells are placed in the buffer solution of a stable paramagnetic probe, and electron spin-resonance microimaging is employed to map out the probe's spin-spin relaxation time (T(2)). This information is directly linked to the concentration of the oxygen molecule. The method is demonstrated with a test sample and with a small amount of live photosynthetic cells (cyanobacteria), under conditions of darkness and light. Spatial resolution of approximately 30 x 30 x 100 microm is demonstrated, with approximately microM oxygen concentration sensitivity and sub-fmol absolute oxygen sensitivity per voxel. The use of electron spin-resonance microimaging for oxygen mapping near cells complements the currently available techniques based on microelectrodes or fluorescence/phosphorescence. Furthermore, with the proper paramagnetic probe, it will also be readily applicable for intracellular oxygen microimaging, a capability which other methods find very difficult to achieve.
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