ArticleBiophysical journal2010
Quantitative imaging of human red blood cells infected with Plasmodium falciparum.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Host iron status and iron supplementation mediate susceptibility to erythrocytic stage Plasmodium falciparum.Nature communications · 2014Trial
- Rosette margination in blood flow during malaria pathogenesis.Biophysical journal · 2026Article
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- Shape-Dependent Structural Order of Red Blood Cells.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Volumetric segmentation of biological cells and subcellular structures for optical diffraction tomography images.Biomedical optics express · 2023Article
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- Babesia, Theileria, Plasmodium and Hemoglobin.Microorganisms · 2022Article
- Biophysical Tools and Concepts Enable Understanding of Asexual Blood Stage Malaria.Frontiers in cellular and infection microbiology · 2022Review
- A Profound Membrane Reorganization Defines Susceptibility ofFrontiers in immunology · 2021Article
- Adhesion between P. falciparum infected erythrocytes and human endothelial receptors follows alternative binding dynamics under flow and febrile conditions.Scientific reports · 2020Article
- Delayed death in the malaria parasite Plasmodium falciparum is caused by disruption of prenylation-dependent intracellular trafficking.PLoS biology · 2019Article
- Multimodal analysis of Plasmodium knowlesi-infected erythrocytes reveals large invaginations, swelling of the host cell, and rheological defects.Cellular microbiology · 2019Article
- Endothelial glycocalyx regulates cytoadherence in Plasmodium falciparum malaria.Journal of the Royal Society, Interface · 2018Article
- Dielectric characterization ofJournal of the Royal Society, Interface · 2018Article
- Cell Swelling Induced by the Antimalarial KAE609 (Cipargamin) and Other PfATP4-Associated Antimalarials.Antimicrobial agents and chemotherapy · 2018Article
- Evidence against a Role of Elevated Intracellular CaBiophysical journal · 2018Article
- Factors Diminishing Cytoadhesion of Red Blood Cells Infected by Plasmodium falciparum in Arterioles.Biophysical journal · 2017Article
- Rolling Adhesion of Schizont Stage Malaria-Infected Red Blood Cells in Shear Flow.Biophysical journal · 2017Article
- Differential time-dependent volumetric and surface area changes and delayed induction of new permeation pathways in P. falciparum-infected hemoglobinopathic erythrocytes.Cellular microbiology · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
During its 48 h asexual reproduction cycle, the malaria parasite Plasmodium falciparum ingests and digests hemoglobin in excess of its metabolic requirements and causes major changes in the homeostasis of the host red blood cell (RBC). A numerical model suggested that this puzzling excess consumption of hemoglobin is necessary for the parasite to reduce the colloidosmotic pressure within the host RBC, thus preventing lysis before completion of its reproduction cycle. However, the validity of the colloidosmotic hypothesis appeared to be compromised by initial conflicts between model volume predictions and experimental observations. Here, we investigated volume and membrane area changes in infected RBCs (IRBCs) using fluorescence confocal microscopy on calcein-loaded RBCs. Substantial effort was devoted to developing and testing a new threshold-independent algorithm for the precise estimation of cell volumes and surface areas to overcome the shortfalls of traditional methods. We confirm that the volume of IRBCs remains almost constant during parasite maturation, suggesting that the reported increase in IRBCs' osmotic fragility results from a reduction in surface area and increased lytic propensity on volume expansion. These results support the general validity of the colloidosmotic hypothesis, settle the IRBC volume debate, and help to constrain the range of parameter values in the numerical model.
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