ArticleBiophysical journal2010
Material properties of lipid microdomains: force-volume imaging study of the effect of cholesterol on lipid microdomain rigidity.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Formation of a Neuronal Membrane Model: A Quartz Crystal Microbalance with Dissipation Monitoring Study.Biomolecules · 2025Article
- Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains.Biophysical journal · 2023Article
- Membrane elasticity modulated by cholesterol in model of porcine eye lens-lipid membrane.Experimental eye research · 2022Article
- Cholesterol-tuned liposomal membrane rigidity directs tumor penetration and anti-tumor effect.Acta pharmaceutica Sinica. B · 2019Article
- Membrane Cholesterol in Skeletal Muscle: A Novel Player in Excitation-Contraction Coupling and Insulin Resistance.Journal of diabetes research · 2017Review
- HIV-1 antibodies and vaccine antigen selectively interact with lipid domains.Biochimica et biophysica acta · 2014Article
- Article
- Multiparametric imaging of biological systems by force-distance curve-based AFM.Nature methods · 2013Review
- Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy.International journal of molecular sciences · 2013Article
- Probing membrane protein interactions with their lipid raft environment using single-molecule tracking and Bayesian inference analysis.PloS one · 2013Article
- Liposomes bearing fibrinogen could potentially interfere with platelet interaction and procoagulant activity.International journal of nanomedicine · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of cholesterol (CHOL) on the material properties of supported lipid bilayers composed of lipid mixtures that mimic the composition of lipid microdomains was studied by force-volume (FV) imaging under near-physiological conditions. These studies were carried out with lipid mixtures of dioleoylphosphatidylcholine, dioleoylphosphatidylserine, and sphingomyelin. FV imaging enabled simultaneous topology and force measurements of sphingomyelin-rich domains (higher domain (HD)) and phospholipid-rich domains (lower domain (LD)), which allowed quantitative measurement of the force needed to puncture the lipid bilayer with or without CHOL. The force required to penetrate the various domains of the bilayer was probed using high- and low-ionic-strength buffers as a function of increasing amounts of CHOL in the bilayer. The progressive addition of CHOL also led to a decreasing height difference between HD and LD. FV imaging further demonstrated a lack of adhesion between the atomic force microscope tip and the HD or LD at loads below the breakthrough force. These results can lead to a better understanding of the role that CHOL plays in the mechanical properties of cellular membranes in modulating membrane rigidity, which has important implications for cellular mechanotransduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.