ArticleBiophysical journal2010
Polymer partitioning and ion selectivity suggest asymmetrical shape for the membrane pore formed by epsilon toxin.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- The Molecular Architecture and Mode of Action ofToxins · 2024Review
- Interaction ofToxins · 2022Article
- Article
- Development of a double-recombinant antibody sandwich ELISA for quantitative detection of epsilon toxoid concentration in inactivated Clostridium perfringens vaccines.BMC veterinary research · 2020Article
- Clostridium perfringens epsilon toxin binds to erythrocyte MAL receptors and triggers phosphatidylserine exposure.Journal of cellular and molecular medicine · 2020Article
- The pore structure of Clostridium perfringens epsilon toxin.Nature communications · 2019Article
- A Novel Panel of Rabbit Monoclonal Antibodies and Their Diverse Applications Including Inhibition ofAntibodies (Basel, Switzerland) · 2018Article
- The Cytotoxicity of Epsilon Toxin from Clostridium perfringens on Lymphocytes Is Mediated by MAL Protein Expression.Molecular and cellular biology · 2018Article
- Cell-free production of pore forming toxins: Functional analysis of thermostable direct hemolysin fromEngineering in life sciences · 2018Article
- F199E substitution reduced toxicity of Clostridium perfringens epsilon toxin by depriving the receptor binding capability.Human vaccines & immunotherapeutics · 2017Article
- Review
- Forces from the Portal Govern the Late-Stage DNA Transport in a Viral DNA Packaging Nanomotor.Biophysical journal · 2016Article
- Immunization with a novel Clostridium perfringens epsilon toxin mutant rETX(Y196E)-C confers strong protection in mice.Scientific reports · 2016Article
- Inhibiting bacterial toxins by channel blockage.Pathogens and disease · 2016Review
- The Myelin and Lymphocyte Protein MAL Is Required for Binding and Activity of Clostridium perfringens ε-Toxin.PLoS pathogens · 2015Article
- Review
- Toxin plasmids of Clostridium perfringens.Microbiology and molecular biology reviews : MMBR · 2013Review
- Obstructing toxin pathways by targeted pore blockage.Chemical reviews · 2012Review
- Inspection of the engineered FhuA ΔC/Δ4L protein nanopore by polymer exclusion.Biophysical journal · 2012Article
- Coenzyme depletion by members of the aerolysin family of pore-forming toxins leads to diminished ATP levels and cell death.Molecular bioSystems · 2012Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Using poly-(ethylene glycol)s of different molecular weights, we probe the channels formed in planar lipid bilayers by epsilon toxin secreted by the anaerobic bacterium Clostridium perfringens. We find that the pore is highly asymmetric. The cutoff size of polymers entering the pore through its opening from the cis side, the side of toxin addition, is approximately 500 Da, whereas the cutoff size for the polymers entering from the trans side is approximately 2300 Da. Comparing these characteristic molecular weights with those reported earlier for OmpF porin and the alpha-Hemolysin channel, we estimate the radii of cis and trans openings as 0.4 nm and 1.0 nm, respectively. The simplest geometry corresponding to these findings is that of a truncated cone. The asymmetry of the pore is also confirmed by measurements of the reversal potential at oppositely directed salt gradients. The moderate anionic selectivity of the channel is salted-out more efficiently when the salt concentration is higher at the trans side of the pore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.