ArticleJournal of the American Chemical Society2010
Toward a general approach for RNA-templated hierarchical assembly of split-proteins.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification.Nature communications · 2025Article
- Luciferase complementation for cellular assays beyond protein-protein interactions.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Enzyme fragment complementation driven by nucleic acid hybridization sans self-labeling protein.Bioorganic chemistry · 2025Article
- Enzyme Fragment Complementation Driven by Nucleic Acid Hybridization.bioRxiv : the preprint server for biology · 2023Article
- A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain.Synthetic and systems biotechnology · 2023Article
- Engineering protein and DNA tools for creating DNA-dependent protein switches.Methods in enzymology · 2022Article
- Expanding RNA binding specificity and affinity of engineered PUF domains.Nucleic acids research · 2018Article
- Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids.Chemical communications (Cambridge, England) · 2017Article
- DNA-based control of protein activity.Chemical communications (Cambridge, England) · 2016Article
- Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation.International journal of molecular sciences · 2015Article
- Labeling native bacterial RNA in live cells.Cell research · 2014Article
- Review
- Engineering RNA-binding proteins for biology.The FEBS journal · 2013Review
- Engineered proteins with Pumilio/fem-3 mRNA binding factor scaffold to manipulate RNA metabolism.The FEBS journal · 2013Review
- Split-protein systems: beyond binary protein-protein interactions.Current opinion in chemical biology · 2011Review
- Turn-on DNA damage sensors for the direct detection of 8-oxoguanine and photoproducts in native DNA.Journal of the American Chemical Society · 2011Article
- Alternate modes of cognate RNA recognition by human PUMILIO proteins.Structure (London, England : 1993) · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to conditionally turn on a signal or induce a function in the presence of a user-defined RNA target has potential applications in medicine and synthetic biology. Although sequence-specific pumilio repeat proteins can target a limited set of ssRNA sequences, there are no general methods for targeting ssRNA with designed proteins. As a first step toward RNA recognition, we utilized the RNA binding domain of argonaute, implicated in RNA interference, for specifically targeting generic 2-nucleotide, 3' overhangs of any dsRNA. We tested the reassembly of a split-luciferase enzyme guided by argonaute-mediated recognition of newly generated nucleotide overhangs when ssRNA is targeted by a designed complementary guide sequence. This approach was successful when argonaute was utilized in conjunction with a pumilio repeat and expanded the scope of potential ssRNA targets. However, targeting any desired ssRNA remained elusive as two argonaute domains provided minimal reassembled split-luciferase. We next designed and tested a second hierarchical assembly, wherein ssDNA guides are appended to DNA hairpins that serve as a scaffold for high affinity zinc fingers attached to split-luciferase. In the presence of a ssRNA target containing adjacent sequences complementary to the guides, the hairpins are brought into proximity, allowing for zinc finger binding and concomitant reassembly of the fragmented luciferase. The scope of this new approach was validated by specifically targeting RNA encoding VEGF, hDM2, and HER2. These approaches provide potentially general design paradigms for the conditional reassembly of fragmented proteins in the presence of any desired ssRNA target.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.