ArticleCytotechnology2010
Investigating the establishment of primary cell culture from different abalone (Haliotis midae) tissues.
Article in Cytotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Development of long-term oyster explant cultures enable sustained tissue-specific activity and reveal novel cellular behaviours.Biochemistry and biophysics reports · 2026Article
- Challenges in cellular agriculture: lessons from Pacific white shrimp, Litopenaeus vannamei.In vitro cellular & developmental biology. Animal · 2025Article
- Mechanisms of HAHV-1 Interaction with Hemocytes inBiology · 2025Article
- Status in molluscan cell line development in last one decade (2010-2020): impediments and way forward.Cytotechnology · 2022Review
- Lipoprotein-induced cell growth and hemocyanin biosynthesis in rhogocytes.Cell and tissue research · 2022Article
- Transcriptome analysis of air-breathing land slug, Incilaria fruhstorferi reveals functional insights into growth, immunity, and reproduction.BMC genomics · 2019Article
- Nacre formation by epithelial cell cultures from mantle of the black-lip pearl oyster, Pinctada margaritifera.In vitro cellular & developmental biology. Animal · 2018Article
- The cell cultures and the use of haemocytes from marine molluscs for ecotoxicology assessment.Cytotechnology · 2016Review
- Primary cell cultures of regenerating holothurian tissues.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures.Cytotechnology · 2013Article
- Molluscan cells in culture: primary cell cultures and cell lines.Canadian journal of zoology · 2013Article
- RNAi assay in primary cells: a new method for gene function analysis in marine bivalve.Molecular biology reports · 2012Article
- Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin.Cytotechnology · 2012Article
- Differential growth-related gene expression in abalone (Haliotis midae).Marine biotechnology (New York, N.Y.) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The abalone, Haliotis midae, is the most valuable commodity in South African aquaculture. The increasing demand for marine shellfish has stimulated research on the biology and physiology of target species in order to improve knowledge on growth, nutritional requirements and pathogen identification. The slow growth rate and long generation time of abalone restrict efficient design of in vivo experiments. Therefore, in vitro systems present an attractive alternative for short term experimentation. The use of marine invertebrate cell cultures as a standardised and controlled system to study growth, endocrinology and disease contributes to the understanding of the biology of economically important molluscs. This paper investigates the suitability of two different H. midae tissues, larval and haemocyte, for establishing primary cell cultures. Cell cultures are assessed in terms of culture initiation, cell yield, longevity and susceptibility to contamination. Haliotis midae haemocytes are shown to be a more feasible tissue for primary cell culture as it could be maintained without contamination more readily than larval cell cultures. The usefulness of short term primary haemocyte cultures is demonstrated here with a growth factor trial. Haemocyte cultures can furthermore be used to relate phenotypic changes at the cellular level to changes in gene expression at the molecular level.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.