Evidence map›Paper›PMID 20680444›Full record

ArticleTropical animal health and production2011

Phylogenetic background and virulence genes of Escherichia coli isolates from colisepticemic and healthy broiler chickens in Iran.

Reza Ghanbarpour, Masoud Sami, Mahmood Salehi, Mahla Ouromiei

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Tropical animal health and production, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Phylogenetic Group ofTheScientificWorldJournal · 2017
    Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Reza GhanbarpourMolecular Microbiology Research Group, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, P.O. Box 7616914111, Kerman, Iran. ghanbar@mail.uk.ac.ir
Masoud Sami
Mahmood Salehi
Mahla Ouromiei

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purposes of this study were to determine the phylogenetic background and the virulence gene profiles of Escherichia coli isolates from colisepticemic and feces of healthy (AFEC) broiler chickens. In this study, 253 E. coli isolates including 141 avian pathogenic E. coli (APEC) and 112 AFEC isolates were examined by PCR. In general, 253 E. coli isolates distributed among group A (51.8%), B1 (15.8%), B2 (8.7%), and D (23.7%). Ten (8.9%) AFEC isolates segregated in to B1 phylo-group and 102 (91.1%) isolates fell into six different phylogenetic subgroups. Distribution of colisepticemic and fecal isolates differed significantly in their assignments to A and B1 phylo-groups. The three most prevalent virulence genes were crl, fimH, and aer in isolates between both groups. The four genetic markers aer, papC, afa, and sfa were detected significantly more often among colisepticemic isolates than in fecal isolates from healthy broilers. The presence of stx ( 2 ) gene in fecal isolates were significantly differs among the colisepticemic isolates. F17 fimbrial family encoding gene and eae gene were detected in APEC and AFEC isolates, respectively. The colisepticemic and fecal isolates possessed the virulence genes were detected in all of the four phylogenetic groups. Several combination patterns of the virulence genes were detected in APEC and AFEC isolates. In colisepticemic isolates the combination of aer, crl, and fimH genes was the most prevalent pattern. None of the examined isolates harbored the cdt, cnf1, ipaH, and stx ( 1 ) virulence gene sequences.

Indexed as

ChickensPhylogenyAnimalsEscherichia coliEscherichia coli InfectionsFecesGenetic MarkersPolymerase Chain ReactionPoultry DiseasesSepsisVirulenceGenetic Markers

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