ArticleJournal of mammary gland biology and neoplasia2010
Isolation of mammary-specific extracellular matrix to assess acute cell-ECM interactions in 3D culture.
Article in Journal of mammary gland biology and neoplasia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Macphatics and PoEMs in Postpartum Mammary Development and Tumor Progression.Journal of mammary gland biology and neoplasia · 2020Review
- Obesity reduces mammary epithelial cell TGFβ1 activity through macrophage-mediated extracellular matrix remodeling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- 3D bioprinted mammary organoids and tumoroids in human mammary derived ECM hydrogels.Acta biomaterialia · 2019Article
- The Effect of Mammary Extracellular Matrix in Controlling Oral and Mammary Cancer CellsAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Opportunities for organoids as new models of aging.The Journal of cell biology · 2018Review
- Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix.Biochemistry and biophysics reports · 2017Article
- Mammary extracellular matrix directs differentiation of testicular and embryonic stem cells to form functional mammary glands in vivo.Scientific reports · 2017Article
- The extracellular matrix: Tools and insights for the "omics" era.Matrix biology : journal of the International Society for Matrix Biology · 2016Review
- Efferocytosis creates a tumor microenvironment supportive of tumor survival and metastasis.Cancer cell & microenvironment · 2015Article
- Collagen architecture in pregnancy-induced protection from breast cancer.Journal of cell science · 2013Article
- Rat mammary extracellular matrix composition and response to ibuprofen treatment during postpartum involution by differential GeLC-MS/MS analysis.Journal of proteome research · 2012Article
- Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells.PloS one · 2012Article
- Mechanisms by which the extracellular matrix and integrin signaling act to regulate the switch between tumor suppression and tumor promotion.Journal of mammary gland biology and neoplasia · 2011Review
- Non-steroidal anti-inflammatory drugs target the pro-tumorigenic extracellular matrix of the postpartum mammary gland.The International journal of developmental biology · 2011Article
- Editorial: The mammary stroma in normal development and function.Journal of mammary gland biology and neoplasia · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies of mammary epithelial cells (MECs) cultured with reconstituted basement membrane proteins derived from EHS tumors have contributed greatly to the understanding of both normal physiology and transformation. Only when plated on such biologically relevant substratum are MECs able to form morphologically correct, differentiated structures, highlighting a critical role for extracellular matrix (ECM) proteins in MEC organization and function. Here, we describe methods modified from the original EHS matrix protocol for isolating tissue-specific ECM from rat mammary glands, and for subsequent use in short-term 3D cell culture models designed to assess acute cell-ECM interactions. Using this protocol, the final matrix is enriched up to 58-fold for ECM proteins such as fibronectin and laminin, while cellular proteins such as GAPDH are reduced 98-fold. We have previously shown that MECs plated in mammary-specific ECM form more elaborate duct-like and alveolar-like structures compared to MECs plated in Matrigel™, demonstrating the biological relevance of tissue-specific ECM. Use of mammary-specific ECM in 3D cell culture models will further our ability to study the intricate interplay between a cell and its microenvironment, and permit identification of modifying factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.