ArticleIntegrative biology : quantitative biosciences from nano to macro2010
Directional decisions during neutrophil chemotaxis inside bifurcating channels.
Article in Integrative biology : quantitative biosciences from nano to macro, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- Leveraging Microphysiological Systems to Facilitate Neutrophil-Based Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Trade-off between branching and polarity controls decision-making during cell migration.Science advances · 2026Article
- Microtubules regulate tissue-level navigation in skin-resident macrophages.Journal of cell science · 2025Article
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- High-throughput co-culture system for analysis of spatiotemporal cell-cell signaling.Biosensors & bioelectronics · 2023Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Leading edge competition promotes context-dependent responses to receptor inputs to resolve directional dilemmas in neutrophil migration.Cell systems · 2023Article
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Multifactorial assessment of neutrophil chemotaxis efficiency from a drop of blood.Journal of leukocyte biology · 2022Article
- Characterization of immune cell migration using microfabrication.Biophysical reviews · 2021Review
- Energetic costs regulated by cell mechanics and confinement are predictive of migration path during decision-making.Nature communications · 2019Article
- In vitro and ex vivo systems at the forefront of infection modeling and drug discovery.Biomaterials · 2019Review
- Super-Low Dose Lipopolysaccharide Dysregulates Neutrophil Migratory Decision-Making.Frontiers in immunology · 2019Article
- Cell Sequence and Mitosis Affect Fibroblast Directional Decision-Making During Chemotaxis in Microfluidic Mazes.Cellular and molecular bioengineering · 2018Article
- Diagnosis of sepsis from a drop of blood by measurement of spontaneous neutrophil motility in a microfluidic assay.Nature biomedical engineering · 2018Article
- Convergent and Divergent Migratory Patterns of Human Neutrophils inside Microfluidic Mazes.Scientific reports · 2018Article
- Nuclear Deformation During Neutrophil Migration at Sites of Inflammation.Frontiers in immunology · 2018Review
- An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood.Journal of visualized experiments : JoVE · 2017Article
- A Haptotaxis Assay for Neutrophils using Optical Patterning and a High-content Approach.Scientific reports · 2017Article
- Chemotaxis and autochemotaxis of self-propelling droplet swimmers.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The directional migration of human neutrophils in classical chemotaxis assays is often described as a "biased random walk" implying significant randomness in speed and directionality. However, these experiments are inconsistent with in vivo observations, where neutrophils can navigate effectively through complex tissue microenvironments towards their targets. Here, we demonstrate a novel biomimetic assay for neutrophil chemotaxis using enclosed microfluidic channels. Remarkably, under these enclosed conditions, neutrophils recapitulate the highly robust and efficient navigation observed in vivo. In straight channels, neutrophils undergo sustained, unidirectional motion towards a chemoattractant source. In more complex maze-like geometries, neutrophils are able to select the most direct route over 90% of the time. Finally, at symmetric bifurcations, neutrophils split their leading edge into two sections and a "tug of war" ensues. The competition between the two new leading edges is ultimately resolved by stochastic, symmetry-breaking behavior. This behavior is suggestive of directional decision-making localized at the leading edge and a signaling role played by the cellular cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.