ArticlePloS one2010
Unlocking short read sequencing for metagenomics.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
86 citing papers in PubMed, 168 citations in OpenAlex.
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- Identification of arsenic oxidizing genes fragment inSaudi journal of biological sciences · 2023Article
- Draft genomes of three closely related low light-adapted Prochlorococcus.BMC genomic data · 2023Article
- Music of metagenomics-a review of its applications, analysis pipeline, and associated tools.Functional & integrative genomics · 2022Review
- Insufficiency in functional genomics studies, data, and applications: A case study of bio-prospecting research in ruminant microbiome.Frontiers in genetics · 2022Review
- RNA Sequencing for Transcript 5'-End Mapping in Mycobacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Impact of host genetics on gut microbiome: Take-home lessons from human and mouse studies.Animal models and experimental medicine · 2020Review
- The development of colitis in Il10Mucosal immunology · 2020Article
- Article
- Can Targeting Non-Contiguous V-Regions With Paired-End Sequencing Improve 16S rRNA-Based Taxonomic Resolution of Microbiomes?: AnFrontiers in genetics · 2019Article
- Longitudinal microbiome profiling reveals impermanence of probiotic bacteria in domestic pigeons.PloS one · 2019Article
- The DNA double-strand "breakome" of mouse spermatids.Cellular and molecular life sciences : CMLS · 2018Article
- Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses.Scientific data · 2018Article
- Tomatidine Is a Lead Antibiotic Molecule That Targets Staphylococcus aureus ATP Synthase Subunit C.Antimicrobial agents and chemotherapy · 2018Article
- Development ofApplied and environmental microbiology · 2017Article
- First draft genome sequence of a strain belonging to theStandards in genomic sciences · 2017Article
- MeFiT: merging and filtering tool for illumina paired-end reads for 16S rRNA amplicon sequencing.BMC bioinformatics · 2016Article
- Mutational landscape of EGFR-, MYC-, and Kras-driven genetically engineered mouse models of lung adenocarcinoma.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Multiplex sequencing of bacterial artificial chromosomes for assembling complex plant genomes.Plant biotechnology journal · 2016Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDifferent high-throughput nucleic acid sequencing platforms are currently available but a trade-off currently exists between the cost and number of reads that can be generated versus the read length that can be achieved. METHODOLOGY/PRINCIPAL
findingsWe describe an experimental and computational pipeline yielding millions of reads that can exceed 200 bp with quality scores approaching that of traditional Sanger sequencing. The method combines an automatable gel-less library construction step with paired-end sequencing on a short-read instrument. With appropriately sized library inserts, mate-pair sequences can overlap, and we describe the SHERA software package that joins them to form a longer composite read. CONCLUSIONS/SIGNIFICANCE: This strategy is broadly applicable to sequencing applications that benefit from low-cost high-throughput sequencing, but require longer read lengths. We demonstrate that our approach enables metagenomic analyses using the Illumina Genome Analyzer, with low error rates, and at a fraction of the cost of pyrosequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.