ArticleJournal of bacteriology2010
Regulation of a novel gene cluster involved in secondary metabolite production in Streptomyces coelicolor.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- A new peucemycin derivative and impacts of peuR and bldA on peucemycin biosynthesis in Streptomyces peucetius.Applied microbiology and biotechnology · 2024Article
- Genus-specific secondary metabolome inSynthetic and systems biotechnology · 2024Article
- Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor.Microbial cell factories · 2024Article
- Structure and Biosynthesis of Hectoramide B, a Linear Depsipeptide from Marine CyanobacteriumACS chemical biology · 2024Article
- Enhancing the Antibiotic Production by Thermophilic Bacteria Isolated from Hot Spring Waters via Ethyl Methanesulfonate Mutagenesis.Antibiotics (Basel, Switzerland) · 2023Article
- 6S-Like scr3559 RNA Affects Development and Antibiotic Production inMicroorganisms · 2021Article
- Submerged fermentation of Streptomyces uncialis providing a biotechnology platform for uncialamycin biosynthesis, engineering, and production.Journal of industrial microbiology & biotechnology · 2021Article
- Identification and enhancing production of a novel macrolide compound in engineeredRSC advances · 2021Article
- Coupled Transcriptomics for Differential Expression Analysis and Determination of Transcription Start Sites: Design and Bioinformatics.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis.Scientific reports · 2019Article
- Aggregation of germlings is a major contributing factor towards mycelial heterogeneity of Streptomyces.Scientific reports · 2016Article
- The Sound of Silence: Activating Silent Biosynthetic Gene Clusters in Marine Microorganisms.Marine drugs · 2015Review
- Development, antibiotic production, and ribosome assembly in Streptomyces venezuelae are impacted by RNase J and RNase III deletion.Journal of bacteriology · 2014Article
- Article
- Integrated metabolomics approach facilitates discovery of an unpredicted natural product suite from Streptomyces coelicolor M145.ACS chemical biology · 2013Article
- A novel nucleoid-associated protein specific to the actinobacteria.Nucleic acids research · 2013Article
- Saccharopolyspora erythraea's genome is organised in high-order transcriptional regions mediated by targeted degradation at the metabolic switch.BMC genomics · 2013Article
- Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibiotic biosynthesis in the streptomycetes is a complex and highly regulated process. Here, we provide evidence for the contribution of a novel genetic locus to antibiotic production in Streptomyces coelicolor. The overexpression of a gene cluster comprising four protein-encoding genes (abeABCD) and an antisense RNA-encoding gene (α-abeA) stimulated the production of the blue-pigmented metabolite actinorhodin on solid medium. Actinorhodin production also was enhanced by the overexpression of an adjacent gene (abeR) encoding a predicted Streptomyces antibiotic regulatory protein (SARP), while the deletion of this gene impaired actinorhodin production. We found the abe genes to be differentially regulated and controlled at multiple levels. Upstream of abeA was a promoter that directed the transcription of abeABCD at a low but constitutive level. The expression of abeBCD was, however, significantly upregulated at a time that coincided with the initiation of aerial development and the onset of secondary metabolism; this expression was activated by the binding of AbeR to four heptameric repeats upstream of a promoter within abeA. Expressed divergently to the abeBCD promoter was α-abeA, whose expression mirrored that of abeBCD but did not require activation by AbeR. Instead, α-abeA transcript levels were subject to negative control by the double-strand-specific RNase, RNase III.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.