ArticleJournal of bacteriology2010
The delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Proteomic profiling ofTurkish journal of biology = Turk biyoloji dergisi · 2025Article
- The Pleiotropic Effects of Carbohydrate-Mediated Growth Rate Modifications inMicroorganisms · 2023Article
- Critical Effect of HMicrobiology spectrum · 2022Article
- Loss ofMicroorganisms · 2022Article
- Structural basis of transcription activation by the global regulator Spx.Nucleic acids research · 2021Article
- Methylation of rRNA as a host defense against rampant group II intron retrotransposition.Mobile DNA · 2021Article
- The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling.Nature communications · 2020Article
- Metagenomics Biomarkers Selected for Prediction of Three Different Diseases in Chinese Population.BioMed research international · 2018Article
- Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't.Molecular oral microbiology · 2017Review
- The ω Subunit Governs RNA Polymerase Stability and Transcriptional Specificity in Staphylococcus aureus.Journal of bacteriology · 2017Article
- A Novel Function of δ Factor from Bacillus subtilis as a Transcriptional Repressor.The Journal of biological chemistry · 2016Article
- Parallel Evolution in Streptococcus pneumoniae Biofilms.Genome biology and evolution · 2016Article
- RpoE is a Putative Antibiotic Resistance Regulator of Salmonella enteric Serovar Typhi.Current microbiology · 2016Article
- Bacillus subtilis δ Factor Functions as a Transcriptional Regulator by Facilitating the Open Complex Formation.The Journal of biological chemistry · 2016Article
- Streptococcus mutans copes with heat stress by multiple transcriptional regulons modulating virulence and energy metabolism.Scientific reports · 2015Article
- Small things considered: the small accessory subunits of RNA polymerase in Gram-positive bacteria.FEMS microbiology reviews · 2015Review
- Biofilm-specific extracellular matrix proteins of nontypeable Haemophilus influenzae.Pathogens and disease · 2014Article
- Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans.The ISME journal · 2014Article
- Integrated transcriptomic and proteomic analysis of the bile stress response in a centenarian-originated probiotic Bifidobacterium longum BBMN68.Molecular & cellular proteomics : MCP · 2014Article
- The δ subunit of RNA polymerase guides promoter selectivity and virulence in Staphylococcus aureus.Infection and immunity · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied biochemical properties of RpoE, a role for this protein in vivo has not been defined in depth. In this study, we show that inactivation of rpoE in the human dental caries pathogen Streptococcus mutans causes impaired growth and loss of important virulence traits, including biofilm formation, resistance to antibiotics, and tolerance to environmental stresses. Complementation of the mutant with rpoE expressed in trans restored its phenotype to wild type. The luciferase fusion reporter showed that rpoE was highly transcribed throughout growth and that acid and hydrogen peroxide stresses repressed rpoE expression. Transcriptome profiling of wild-type and ΔrpoE cells in the exponential and early stationary phase of growth, under acid and hydrogen peroxide stress and under both stresses combined, revealed that genes involved in histidine synthesis, malolactic fermentation, biofilm formation, and antibiotic resistance were downregulated in the ΔrpoE mutant under all conditions. Moreover, the loss of RpoE resulted in dramatic changes in transport and metabolism of carbohydrates and amino acids. Interestingly, differential expression, mostly upregulation, of 330 noncoding regions was found. In conclusion, this study demonstrates that RpoE is an important global modulator of gene expression in S. mutans which is required for optimal growth and environmental adaptation.
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