ArticleApplied and environmental microbiology2010
The chitinolytic activity of Listeria monocytogenes EGD is regulated by carbohydrates but also by the virulence regulator PrfA.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- Bacterial Chitinases and Their Role in Human Infection.Infection and immunity · 2023Review
- Metabolism of Poly-β1,4-Journal of bacteriology · 2021Article
- Genomic Differences between Listeria monocytogenes EGDe Isolates Reveal Crucial Roles for SigB and Wall Rhamnosylation in Biofilm Formation.Journal of bacteriology · 2020Article
- Chitin Attenuates Expression ofFrontiers in microbiology · 2020Article
- Chitinase Expression in Listeria monocytogenes Is Influenced byApplied and environmental microbiology · 2017Article
- Profiling of Virulence Determinants in Cronobacter sakazakii Isolates from Different Plant and Environmental Commodities.Current microbiology · 2017Article
- Transcriptomic Analysis of the Adaptation ofFrontiers in microbiology · 2017Article
- Genomic potential for polysaccharide deconstruction in bacteria.Applied and environmental microbiology · 2015Article
- Community-wide transcriptome of the oral microbiome in subjects with and without periodontitis.The ISME journal · 2014Article
- Small RNA functions in carbon metabolism and virulence of enteric pathogens.Frontiers in cellular and infection microbiology · 2014Review
- Chitinase expression in Listeria monocytogenes is positively regulated by the Agr system.PloS one · 2014Article
- Microdiversity of extracellular enzyme genes among sequenced prokaryotic genomes.The ISME journal · 2013Article
- Article
- Excretome of the chitinolytic bacterium Clostridium paraputrificum J4.Folia microbiologica · 2012Article
- Optimizing the balance between host and environmental survival skills: lessons learned from Listeria monocytogenes.Future microbiology · 2012Review
- Article
- Contribution of chitinases to Listeria monocytogenes pathogenesis.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin, an insoluble polymer of N-acetyl-D-glucosamine (GlcNAc), is one of the most abundant carbohydrate polymers in marine and terrestrial environments. Chitin hydrolysis by Listeria monocytogenes depends on two chitinase-encoding genes, chiA and chiB, and the aim of this study was to investigate their regulation. Chitin induces the expression of both chitinases in late exponential growth phase, and chiA but not chiB is furthermore induced by the monomer GlcNAc. Furthermore, their expression is subjected to catabolite control. Chitinases expressed by bacterial pathogens have proven to be important not only for nutrient acquisition and environmental survival but also for infecting animals and humans. Interestingly, the central L. monocytogenes virulence gene regulator, PrfA, is required for the chitinolytic phenotype, as chitinase activity was significantly reduced in prfA mutant cells compared to its level in wild-type cells. In agreement with this, Northern blot analysis showed that the amounts of chiA and chiB transcripts upon induction by chitin were significantly lower in the prfA mutant than in the wild type. The chitinolytic activity and chiA and chiB expression were reduced in the absence of the sigB gene, indicating that σ(B) is also important for the production of chitinases. The chiA, chiB, and chiA chiB mutants were not impaired for in vitro adhesion and invasion in epithelial cell lines, but the chiA chiB double mutant showed less survival ability in a chitin-enriched medium. The regulation of chitinolytic activity in L. monocytogenes is complex, and taken together, the results indicate that the biological role of this activity may not be limited to the external environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.