ArticleRNA (New York, N.Y.)2010
Translational competence of ribosomes released from a premature termination codon is modulated by NMD factors.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 49 citations in OpenAlex.
- Ataluren for drug-resistant epilepsy in nonsense variant-mediated Dravet syndrome and CDKL5 deficiency disorder.Annals of clinical and translational neurology · 2021Trial
- Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes.The Journal of biological chemistry · 2024Article
- Genetic screens in Saccharomyces cerevisiae identify a role for 40S ribosome recycling factors Tma20 and Tma22 in nonsense-mediated decay.G3 (Bethesda, Md.) · 2024Article
- Ribosome-bound Upf1 forms distinct 80S complexes and conducts mRNA surveillance.RNA (New York, N.Y.) · 2022Article
- Features and factors that dictate if terminating ribosomes cause or counteract nonsense-mediated mRNA decay.The Journal of biological chemistry · 2022Review
- The G3BP1-UPF1-Associated Long Non-Coding RNA CALA Regulates RNA Turnover in the Cytoplasm.Non-coding RNA · 2022Article
- Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames.Nucleic acids research · 2021Article
- Inhibition of post-termination ribosome recycling at premature termination codons in UPF1 ATPase mutants.eLife · 2020Article
- How to get away with nonsense: Mechanisms and consequences of escape from nonsense-mediated RNA decay.Wiley interdisciplinary reviews. RNA · 2020Review
- Nonsense-mediated mRNA decay involves two distinct Upf1-bound complexes.The EMBO journal · 2018Article
- New insights into the interplay between the translation machinery and nonsense-mediated mRNA decay factors.Biochemical Society transactions · 2018Review
- Upf proteins: highly conserved factors involved in nonsense mRNA mediated decay.Molecular biology reports · 2018Review
- Dual function of UPF3B in early and late translation termination.The EMBO journal · 2017Article
- High-resolution profiling of NMD targets in yeast reveals translational fidelity as a basis for substrate selection.RNA (New York, N.Y.) · 2017Article
- An RNA decay factor wears a new coat: UPF3B modulates translation termination.F1000Research · 2017Review
- Exon junction complex proteins bind nascent transcripts independently of pre-mRNA splicing ineLife · 2016Article
- Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain.RNA (New York, N.Y.) · 2015Article
- Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.Nucleic acids research · 2015Article
- Review
- Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.Annual review of genetics · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In addition to their well-documented roles in the promotion of nonsense-mediated mRNA decay (NMD), yeast Upf proteins (Upf1, Upf2/Nmd2, and Upf3) also manifest translational regulatory functions, at least in vitro, including roles in premature translation termination and subsequent reinitiation. Here, we find that all upf Delta strains also fail to reinitiate translation after encountering a premature termination codon (PTC) in vivo, a result that led us to seek a unifying mechanism for all of these translation phenomena. Comparisons of the in vitro translational activities of wild-type (WT) and upf1 Delta extracts were utilized to test for a Upf1 role in post-termination ribosome reutilization. Relative to WT extracts, non-nucleased extracts lacking Upf1 had approximately twofold decreased activity for the translation of synthetic CAN1/LUC mRNA, a defect paralleled by fewer ribosomes per mRNA and reduced efficiency of the 60S joining step at initiation. These deficiencies could be complemented by purified FLAG-Upf1, or 60S subunits, and appeared to reflect diminished cycling of ribosomes from endogenous PTC-containing mRNAs to exogenously added synthetic mRNA in the same extracts. This hypothesis was tested, and supported, by experiments in which nucleased WT or upf1 Delta extracts were first challenged with high concentrations of synthetic mRNAs that were templates for either normal or premature translation termination and then assayed for their capacity to translate a normal mRNA. Our results indicate that Upf1 plays a key role in a mechanism coupling termination and ribosome release at a PTC to subsequent ribosome reutilization for another round of translation initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.