ArticleBiochemical and biophysical research communications2010
Genome-to-function characterization of novel fungal P450 monooxygenases oxidizing polycyclic aromatic hydrocarbons (PAHs).
Article in Biochemical and biophysical research communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 121 citations in OpenAlex.
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- Phenanthrene-Induced Cytochrome P450 Genes and Phenanthrene Tolerance Associated withPlants (Basel, Switzerland) · 2024Article
- DNA adduct formation in Saccharomyces cerevisiae following exposure to environmental pollutants, as in vivo model for molecular toxicity studies.World journal of microbiology & biotechnology · 2024Article
- White Rot Fungi as Tools for the Bioremediation of Xenobiotics: A Review.Journal of fungi (Basel, Switzerland) · 2024Review
- Synergistic lignin degradation between Phanerochaete chrysosporium and Fenton chemistry is mediated through iron cycling and ligninolytic enzyme induction.The Science of the total environment · 2023Article
- Biodegradation of Benzo[a]pyrene by a White-Rot FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Fungal bioproducts for petroleum hydrocarbons and toxic metals remediation: recent advances and emerging technologies.Bioprocess and biosystems engineering · 2023Review
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- An insight on microbial degradation of benzo[a]pyrene: current status and advances in research.World journal of microbiology & biotechnology · 2022Review
- Preparative Production of Functionalized (Biomolecules · 2022Article
- Effects of Allelochemicals, Soil Enzyme Activities, and Environmental Factors on Rhizosphere Soil Microbial Community ofMicroorganisms · 2022Article
- Late-Stage Functionalisation of Polycyclic (Biomolecules · 2021Article
- Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family.Applied microbiology and biotechnology · 2021Article
- Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil.Scientific reports · 2021Article
- Reductive Cytochrome P450 Reactions and Their Potential Role in Bioremediation.Frontiers in microbiology · 2021Review
- Article
- A Fungal P450 Enzyme from Thanatephorus cucumeris with Steroid Hydroxylation Capabilities.Applied and environmental microbiology · 2018Article
- Recombinant production of eukaryotic cytochrome P450s in microbial cell factories.Bioscience reports · 2018Review
- Molecular perspectives and recent advances in microbial remediation of persistent organic pollutants.Environmental science and pollution research international · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Fungi, particularly the white rot basidiomycetes, have an extraordinary capability to degrade and/or mineralize (to CO(2)) the recalcitrant fused-ring high molecular weight (4 aromatic-rings) polycyclic aromatic hydrocarbons (HMW PAHs). Despite over 30years of research demonstrating involvement of P450 monooxygenation reactions in fungal metabolism of HMW PAHs, specific P450 monooxygenases responsible for oxidation of these compounds are not yet known. Here we report the first comprehensive identification and functional characterization of P450 monooxygenases capable of oxidizing different ring-size PAHs in the model white rot fungus Phanerochaete chrysosporium using a successful genome-to-function strategy. In a genome-wide P450 microarray screen, we identified six PAH-responsive P450 genes (Pc-pah1-Pc-pah6) inducible by PAHs of varying ring size, namely naphthalene, phenanthrene, pyrene, and benzo(a)pyrene (BaP). Using a co-expression strategy, cDNAs of the six Pc-Pah P450s were cloned and expressed in Pichia pastoris in conjunction with the homologous P450 oxidoreductase (Pc-POR). Each of the six recombinant P450 monooxygenases showed PAH-oxidizing activity albeit with varying substrate specificity towards PAHs (3-5 rings). All six P450s oxidized pyrene (4-ring) into two monohydroxylated products. Pc-Pah1 and Pc-Pah3 oxidized BaP (5-ring) to 3-hydroxyBaP whereas Pc-Pah4 and Pc-Pah6 oxidized phenanthrene (3-ring) to 3-, 4-, and 9-phenanthrol. These PAH-oxidizing P450s (493-547 aa) are structurally diverse and novel considering their low overall homology (12-23%) to mammalian counterparts. To our knowledge, this is the first report on specific fungal P450 monooxygenases with catalytic activity toward environmentally persistent and highly toxic HMW PAHs.
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