ArticleJournal of insect science (Online)2010
Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress.
Article in Journal of insect science (Online), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Enhancing foot-and-mouth disease virus thermostability with a VP1 substitution selected by host HSP60 inhibition.Applied microbiology and biotechnology · 2026Article
- Heat Shock Proteins (Hsps) in Cellular Homeostasis: A Promising Tool for Health Management in Crustacean Aquaculture.Life (Basel, Switzerland) · 2022Review
- Article
- Characterization of an inducible HSP70 gene in Chilo suppressalis and expression in response to environmental and biological stress.Cell stress & chaperones · 2020Article
- Identification of HSP70 gene in Corythucha ciliata and its expression profiles under laboratory and field thermal conditions.Cell stress & chaperones · 2018Article
- Identification and Functional Analysis of the First Aquaporin from Striped Stem Borer,Frontiers in physiology · 2018Article
- Characteristics and expression of genes encoding two small heat shock protein genes lacking introns from Chilo suppressalis.Cell stress & chaperones · 2018Article
- Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae).Experimental & applied acarology · 2015Article
- Five small heat shock protein genes from Chilo suppressalis: characteristics of gene, genomic organization, structural analysis, and transcription profiles.Cell stress & chaperones · 2014Article
- Parasitization by Cotesia chilonis influences gene expression in fatbody and hemocytes of Chilo suppressalis.PloS one · 2013Article
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Authors and funding
4 authors.
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Abstract
Heat shock protein 60 is an important chaperonin. In this paper, hsp60 of the stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), was cloned by RT-PCR and rapid amplification of cDNA end (RACE) reactions. The full length cDNA of hsp6 degrees Consisted of 2142 bp, with an ORF of 1719 bp, encoding 572 amino acid residues, with a 5'UTR of 158 bp and a 3'UTR of 265 bp. Cluster analysis confirmed that the deduced amino acid sequence shared high identity with the reported sequences from other insects (77%-86%). To investigate whether hsp60 in C. suppressalis responds to thermal stress, the expression levels of hsp60 mRNA in larval haemocytes across temperature gradients from 31 to 39 degrees C were analysed by real-time quantitative PCR. There was no significant difference for hsp60 expression from 28 to 31 degrees C. he temperatures for maximal induction of hsp60 expression in haemocytes was close to 36 degrees C. Hsp60 expression was observed by using flow cytometry. These results revealed that thermal stress significantly induced hsp60 expression and Hsp60 synthesis in larval haemocytes, and the expression profiles of Hsp60 at the mRNA and protein levels were in high agreement with each other from 33 to 39 degrees C.
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