ArticleTissue engineering. Part A2010
Chondrogenesis and mineralization during in vitro culture of human mesenchymal stem cells on three-dimensional woven scaffolds.
Article in Tissue engineering. Part A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review.Journal of orthopaedic surgery and research · 2017Pooled it
- Potential of gelatin hydrogel nonwoven fabrics (Genocel) as a skin substitute in a diabetic mouse skin defect model.Regenerative therapy · 2024Article
- Materials Suitable for Osteochondral Regeneration.ACS omega · 2024Review
- Development of gelatin hydrogel nonwoven fabrics (Genocel®) as a novel skin substitute in murine skin defects.Regenerative therapy · 2022Article
- Functional tissue engineering of articular cartilage for biological joint resurfacing-The 2021 Elizabeth Winston Lanier Kappa Delta Award.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Assembled Cell-Decorated Collagen (AC-DC) Fiber Bioprinted Implants with Musculoskeletal Tissue Properties Promote Functional Recovery in Volumetric Muscle Loss.Advanced healthcare materials · 2022Article
- Effect of different aged cartilage ECM on chondrogenesis of BMSCsRegenerative biomaterials · 2020Article
- Chondrogenic, hypertrophic, and osteochondral differentiation of human mesenchymal stem cells on three-dimensionally woven scaffolds.Journal of tissue engineering and regenerative medicine · 2019Article
- High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Genetic Engineering of Mesenchymal Stem Cells for Differential Matrix Deposition on 3D Woven Scaffolds.Tissue engineering. Part A · 2018Article
- A Modular Strategy to Engineer Complex Tissues and Organs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Bioreactor mechanically guided 3D mesenchymal stem cell chondrogenesis using a biocompatible novel thermo-reversible methylcellulose-based hydrogel.Scientific reports · 2017Article
- The Use of Silk as a Scaffold for Mature, Sustainable Unilocular Adipose 3D Tissue Engineered Systems.Advanced healthcare materials · 2016Article
- Textile Technologies and Tissue Engineering: A Path Toward Organ Weaving.Advanced healthcare materials · 2016Review
- Strategies for improving the physiological relevance of human engineered tissues.Trends in biotechnology · 2015Review
- A surface-modified poly(ɛ-caprolactone) scaffold comprising variable nanosized surface-roughness using a plasma treatment.Tissue engineering. Part C, Methods · 2014Article
- Chondrogenic differentiation of mesenchymal stem cells: challenges and unfulfilled expectations.Tissue engineering. Part B, Reviews · 2014Review
- Stem cell therapies for knee cartilage repair: the current status of preclinical and clinical studies.The American journal of sports medicine · 2014Review
- Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.Tissue engineering. Part A · 2014Article
- Clinical translation of stem cells: insight for cartilage therapies.Critical reviews in biotechnology · 2014Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Human mesenchymal stem cells (hMSCs) and three-dimensional (3D) woven poly(ɛ-caprolactone) (PCL) scaffolds are promising tools for skeletal tissue engineering. We hypothesized that in vitro culture duration and medium additives can individually and interactively influence the structure, composition, mechanical, and molecular properties of engineered tissues based on hMSCs and 3D poly(ɛ-caprolactone). Bone marrow hMSCs were suspended in collagen gel, seeded on scaffolds, and cultured for 1, 21, or 45 days under chondrogenic and/or osteogenic conditions. Structure, composition, biomechanics, and gene expression were analyzed. In chondrogenic medium, cartilaginous tissue formed by day 21, and hypertrophic mineralization was observed in the newly formed extracellular matrix at the interface with underlying scaffold by day 45. Glycosaminoglycan, hydroxyproline, and calcium contents, and alkaline phosphatase activity depended on culture duration and medium additives, with significant interactive effects (all p < 0.0001). The 45-day constructs exhibited mechanical properties on the order of magnitude of native articular cartilage (aggregate, Young's, and shear moduli of 0.15, 0.12, and 0.033 MPa, respectively). Gene expression was characteristic of chondrogenesis and endochondral bone formation, with sequential regulation of Sox-9, collagen type II, aggrecan, core binding factor alpha 1 (Cbfα1)/Runx2, bone sialoprotein, bone morphogenetic protein-2, and osteocalcin. In contrast, osteogenic medium produced limited osteogenesis. Long-term culture of hMSC on 3D scaffolds resulted in chondrogenesis and regional mineralization at the interface between soft, newly formed engineered cartilage, and stiffer underlying scaffold. These findings merit consideration when developing grafts for osteochondral defect repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.