ArticleJournal of biomedicine & biotechnology2010
Multiple-locus variable-number tandem repeat analysis of Vibrio cholerae in comparison with pulsed field gel electrophoresis and virulotyping.
Article in Journal of biomedicine & biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Antimicrobial susceptibility and virulence genes of clinical and environmental isolates ofPeerJ · 2019Article
- Genome Wide Analysis for Rapid Identification of Vibrio Species.Indian journal of microbiology · 2015Article
- A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors.World journal of microbiology & biotechnology · 2015Article
- Molecular tools in understanding the evolution of Vibrio cholerae.Frontiers in microbiology · 2015Review
- Limitations to estimating bacterial cross-species transmission using genetic and genomic markers: inferences from simulation modeling.Evolutionary applications · 2014Article
- Genome sequence and comparative genomics analysis of a Vibrio cholerae O1 strain isolated from a cholera patient in Malaysia.Journal of bacteriology · 2012Article
- Outbreak-associated Vibrio cholerae genotypes with identical pulsotypes, Malaysia, 2009.Emerging infectious diseases · 2012Article
- Multi-locus variable number tandem repeat analysis of 7th pandemic Vibrio cholerae.BMC microbiology · 2012Article
- Housekeeping gene sequencing and multilocus variable-number tandem-repeat analysis to identify subpopulations within Pseudomonas syringae pv. maculicola and Pseudomonas syringae pv. tomato that correlate with host specificity.Applied and environmental microbiology · 2012Article
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3 authors.
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Abstract
Molecular analysis of Malaysian Vibrio cholerae was carried out using a multiple-locus variable-number tandem repeat analysis (MLVA) assay based on 7 loci of V. cholerae. The discriminatory ability of the assay was compared with pulsed-field gel electrophoresis (PFGE) using 43 Malaysian V. cholerae isolated from various sources. In addition, the virulotypes of the strains were determined. Based on MLVA, 38 allelic profiles were obtained (F = 0.63) while PFGE generated 35 pulsotypes (F = 0.71). Simpson's index of diversity for different VNTR loci ranged from 0.59 to 0.92. The combined loci increased the discriminatory index to 0.99 which was comparable with PFGE (D = 0.99). Most of the environmental non-O1/non-O139 strains harbored rtxA, rstR, toxR, and hlyA only, and the virulotype of this serogroup was significantly different (P < .01) from clinical/environmental O1 and environmental O139 strains. In conclusion, the MLVA assay developed in this study was a useful genotyping tool with comparable discriminatory power with PFGE. In addition, the combination of the two approaches can further distinguish the strains from different sources and geographical regions of isolation.
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