ArticleNature protocols2010
Derivation and characterization of haploid embryonic stem cell cultures in medaka fish.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Established and emerging new approach methodologies in neuroscience.Frontiers in neuroscience · 2025Review
- Characterizing Marine Medaka (Animals : an open access journal from MDPI · 2024Article
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- TGFβ inhibition and mesenchymal to epithelial transition initiation by Xenopus egg extract: first steps towards early reprogramming in fish somatic cell.Scientific reports · 2023Article
- Knockout ofInternational journal of molecular sciences · 2023Article
- Feeder cell-dependent primary culture of single blastula-derived embryonic cell lines from marine medaka (Oryzias dancena).In vitro cellular & developmental biology. Animal · 2022Article
- Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish,Cells · 2022Article
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- Capsid protein from red-spotted grouper nervous necrosis virus induces incomplete autophagy by inactivating the HSP90ab1-AKT-MTOR pathway.Zoological research · 2022Article
- Defining the Pluripotent Marker Genes for Identification of Teleost Fish Cell Pluripotency During Reprogramming.Frontiers in genetics · 2022Article
- Article
- Screening for Antiviral Medaka Haploid Embryonic Stem Cells by Genome Wide Mutagenesis.Marine biotechnology (New York, N.Y.) · 2019Article
- Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells.Biology open · 2018Article
- Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line.PloS one · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem (ES) cells are pluripotent cells capable of differentiation into various cell types. Haploid ES cells elegantly combine the advantages of haploidy and pluripotency and offer a unique in vitro system for genetic analyses of molecular, cellular and developmental events in various cell lineages. Our recent success in generating haploid ES cell lines from gynogenetic embryos of the medaka fish suggests that haploidy can support ES cell derivation and maintenance in a vertebrate. In this study, we present a step-by-step protocol for derivation and characterization of medaka haploid ES cells. We have used this procedure to produce three haploid ES cell lines from five primary cultures. It takes about 15 weeks to generate stable cultures, 5-8 weeks to obtain pure haploid cells and 5-6 weeks to characterize ES cells in vitro and in vivo.
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