ArticlePlant physiology2010
Extracellular nucleotides elicit cytosolic free calcium oscillations in Arabidopsis.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 127 citations in OpenAlex.
- Defective cuticle-derived signals enhance extracellular ATP response and plant immunity.The New phytologist · 2026Article
- Jasmonate primes plant responses to extracellular ATP.The Plant journal : for cell and molecular biology · 2025Article
- Extracellular ATP: an emerging multifaceted regulator of plant fitness.Plant biotechnology journal · 2025Review
- ATP Synthase Members of Chloroplasts and Mitochondria in Rubber Trees (Plants (Basel, Switzerland) · 2025Article
- Differential root zone secretions and the role of root border cells in rhizosphere manipulation.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2025Review
- Understanding the mechanobiology of phytoacoustics through molecular Lens: Mechanisms and future perspectives.Journal of advanced research · 2024Review
- CPK28 is a modulator of purinergic signaling in plant growth and defense.The Plant journal : for cell and molecular biology · 2024Article
- ATP homeostasis and signaling in plants.Plant communications · 2024Review
- Extracellular Self- and Non-Self DNA Involved in Damage Recognition in the Mistletoe Parasitism of Mesquite Trees.International journal of molecular sciences · 2023Article
- The intrinsically disordered C-terminus of purinoceptor P2K1 fine-tunes plant responses to extracellular ATP.FEBS letters · 2023Article
- A histochemical reporter system to study extracellular ATP response in plants.Frontiers in plant science · 2023Article
- Spatial origin of the extracellular ATP-induced cytosolic calcium signature in Arabidopsis thaliana roots: wave formation and variation with phosphate nutrition.Plant biology (Stuttgart, Germany) · 2022Article
- The involvement of extracellular ATP in regulating the stunted growth of Arabidopsis plants by repeated wounding.BMC plant biology · 2022Article
- Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis.The New phytologist · 2022Article
- CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments.Plant physiology · 2022Article
- Cyclic nucleotide-gated ion channel 6 is involved in extracellular ATP signaling and plant immunity.The Plant journal : for cell and molecular biology · 2022Article
- Article
- Activation of the plant mevalonate pathway by extracellular ATP.Nature communications · 2022Article
- Crosstalk between Calcium and ROS Signaling during Flg22-Triggered Immune Response inPlants (Basel, Switzerland) · 2021Article
- Illuminating the hidden world of calcium ions in plants with a universe of indicators.Plant physiology · 2021Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular ATP induces a rise in the level of cytosolic free calcium ([Ca(2+)](cyt)) in plant cells. To expand our knowledge about the function of extracellular nucleotides in plants, the effects of several nucleotide analogs and pharmacological agents on [Ca(2+)](cyt) changes were studied using transgenic Arabidopsis (Arabidopsis thaliana) expressing aequorin or the fluorescence resonance energy transfer-based Ca(2+) sensor Yellow Cameleon 3.6. Exogenously applied CTP caused elevations in [Ca(2+)](cyt) that displayed distinct time- and dose-dependent kinetics compared with the purine nucleotides ATP and GTP. The inhibitory effects of antagonists of mammalian P2 receptors and calcium influx inhibitors on nucleotide-induced [Ca(2+)](cyt) elevations were distinct between CTP and purine nucleotides. These results suggest that distinct recognition systems may exist for the respective types of nucleotides. Interestingly, a mutant lacking the heterotrimeric G protein Gβ-subunit exhibited a remarkably higher [Ca(2+)](cyt) elevation in response to all tested nucleotides in comparison with the wild type. These data suggest a role for Gβ in negatively regulating extracellular nucleotide signaling and point to an important role for heterotrimeric G proteins in modulating the cellular effects of extracellular nucleotides. The addition of extracellular nucleotides induced multiple temporal [Ca(2+)](cyt) oscillations, which could be localized to specific root cells. The oscillations were attenuated by a vesicle-trafficking inhibitor, indicating that the oscillations likely require ATP release via exocytotic secretion. The results reveal new molecular details concerning extracellular nucleotide signaling in plants and the importance of fine control of extracellular nucleotide levels to mediate specific plant cell responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.