ArticleNucleic acids research2010
Arrest of human mitochondrial RNA polymerase transcription by the biological aldehyde adduct of DNA, M1dG.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Accumulation of 8-oxodG within the human mitochondrial genome positively associates with transcription.NAR genomics and bioinformatics · 2023Article
- Mitochondrial DNA damage as driver of cellular outcomes.American journal of physiology. Cell physiology · 2022Review
- Mitochondria in the signaling pathways that control longevity and health span.Ageing research reviews · 2019Review
- Oxidative stress increases M1dG, a major peroxidation-derived DNA adduct, in mitochondrial DNA.Nucleic acids research · 2018Article
- 3-(2-deoxy-β-d-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine adducts of workers exposed to asbestos fibers.Toxicology letters · 2017Article
- Formaldehyde-induced toxicity in the nasal epithelia of workers of a plastic laminate plant.Toxicology research · 2016Article
- Malondialdehyde Epitopes as Targets of Immunity and the Implications for Atherosclerosis.Advances in immunology · 2016Review
- Mitochondrial DNA damage induced autophagy, cell death, and disease.Frontiers in bioscience (Landmark edition) · 2016Review
- Exposure to mitochondrial genotoxins and dopaminergic neurodegeneration in Caenorhabditis elegans.PloS one · 2014Article
- Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal.Oxidative medicine and cellular longevity · 2014Review
- Mitochondria as a target of environmental toxicants.Toxicological sciences : an official journal of the Society of Toxicology · 2013Review
- Exocycilic DNA Adducts in a Murine Model of Non-alcoholic Steatohepatitis.Journal of carcinogenesis & mutagenesis · 2013Article
- LRPPRC/SLIRP suppresses PNPase-mediated mRNA decay and promotes polyadenylation in human mitochondria.Nucleic acids research · 2012Article
- Mitochondrial DNA damage and its consequences for mitochondrial gene expression.Biochimica et biophysica actaReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The biological aldehydes, malondialdehyde and base propenal, react with DNA to form a prevalent guanine adduct, M(1)dG. The exocyclic ring of M(1)dG opens to the acyclic N(2)-OPdG structure when paired with C but remains closed in single-stranded DNA or when mispaired with T. M(1)dG is a target of nucleotide excision repair (NER); however, NER is absent in mitochondria. An in vitro transcription system with purified human mitochondrial RNA polymerase (POLRMT) and transcription factors, mtTFA and mtTFB2, was used to determine the effect of M(1)dG on POLRMT elongation. DNA templates contained a single adduct opposite either C or T downstream of either the light-strand (LSP) or heavy-strand (HSP1) promoter for POLRMT. M(1)dG in the transcribed strand arrested 60-90% POLRMT elongation complexes with greater arrest by the adduct when opposite T. POLRMT was more sensitive to N(2)-OPdG and M(1)dG after initiation at LSP, which suggests promoter-specific differences in the function of POLRMT complexes. A closed-ring analog of M(1)dG, PdG, blocked ≥95% of transcripts originating from either promoter regardless of base pairing, and the transcripts remained associated with POLRMT complexes after stalling at the adduct. This work suggests that persistent M(1)dG adducts in mitochondrial DNA hinder the transcription of mitochondrial genes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.