ArticleBMC medical genomics2010
Regulation of early signaling and gene expression in the alpha-particle and bystander response of IMR-90 human fibroblasts.
Article in BMC medical genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 43 citations in OpenAlex.
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- Radiation Type- and Dose-Specific Transcriptional Responses across Healthy and Diseased Mammalian Tissues.Antioxidants (Basel, Switzerland) · 2022Article
- The transcriptomic revolution and radiation biology.International journal of radiation biology · 2022Article
- An Integrated Preprocessing Approach for Exploring Single-Cell Gene Expression in Rare Cells.Scientific reports · 2019Article
- Targeted and Off-Target (Bystander and Abscopal) Effects of Radiation Therapy: Redox Mechanisms and Risk/Benefit Analysis.Antioxidants & redox signaling · 2018Review
- Changes in gene expression as one of the key mechanisms involved in radiation-induced bystander effect.Biomedical reports · 2018Review
- Identification of differentially expressed genes and pathways in mice exposed to mixed field neutron/photon radiation.BMC genomics · 2018Article
- Global Gene Expression Response in Mouse Models of DNA Repair Deficiency after Gamma Irradiation.Radiation research · 2018Article
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- 50 Years of the Radiological Research Accelerator Facility (RARAF).Radiation research · 2017Article
- Comparison of gene expression response to neutron and x-ray irradiation using mouse blood.BMC genomics · 2017Article
- Bystander signaling via oxidative metabolism.OncoTargets and therapy · 2017Review
- Transcriptome Alterations In X-Irradiated Human Gingiva Fibroblasts.Health physics · 2016Article
- Evaluating biomarkers to model cancer risk post cosmic ray exposure.Life sciences in space research · 2016Review
- The role of protein kinase C alpha translocation in radiation-induced bystander effect.Scientific reports · 2016Article
- High dose bystander effects in spatially fractionated radiation therapy.Cancer letters · 2015Review
- Radiation-induced glioblastoma signaling cascade regulates viability, apoptosis and differentiation of neural stem cells (NSC).Apoptosis : an international journal on programmed cell death · 2014Article
- Identification of gene expression biomarkers for predicting radiation exposure.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe existence of a radiation bystander effect, in which non-irradiated cells respond to signals from irradiated cells, is well established. To understand early signaling and gene regulation in bystander cells, we used a bio-informatics approach, measuring global gene expression at 30 minutes and signaling pathways between 30 minutes and 4 hours after exposure to alpha-particles in IMR-90 fibroblasts.
methodsWe used whole human genome microarrays and real time quantitative PCR to measure and validate gene expression. Microarray analysis was done using BRB-Array Tools; pathway and ontology analyses were done using Ingenuity Pathway Analysis and PANTHER, respectively. We studied signaling in irradiated and bystander cells using immunoblotting and semi-quantitative image analysis.
resultsGene ontology suggested signal transduction and transcriptional regulation responding 30 minutes after treatment affected cell structure, motility and adhesion, and interleukin synthesis. We measured time-dependent expression of genes controlled by the NF-kappaB pathway; matrix metalloproteinases 1 and 3; chemokine ligands 2, 3 and 5 and interleukins 1beta, 6 and 33. There was an increased response of this set of genes 30 minutes after treatment and another wave of induction at 4 hours. We investigated AKT-GSK3beta signaling and found both AKT and GSK3beta are hyper-phosphorylated 30 minutes after irradiation and this effect is maintained through 4 hours. In bystander cells, a similar response was seen with a delay of 30 minutes. We proposed a network model where the observed decrease in phosphorylation of beta-catenin protein after GSK3beta dependent inactivation can trigger target gene expression at later times after radiation exposure
conclusionsThese results are the first to show that the radiation induced bystander signal induces a widespread gene expression response at 30 minutes after treatment and these changes are accompanied by modification of signaling proteins in the PI3K-AKT-GSK3beta pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.