ArticleJournal of thrombosis and haemostasis : JTH2010
Differential phosphorylation of myosin light chain (Thr)18 and (Ser)19 and functional implications in platelets.
Article in Journal of thrombosis and haemostasis : JTH, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Regulation of platelet contractility by agonists present across a thrombus.Blood advances · 2026Article
- Contractility defects hinder glycoprotein VI-mediated platelet activation and affect platelet functions beyond clot contraction.Research and practice in thrombosis and haemostasis · 2024Article
- Histopathogenesis of bone- and soft-tissue tumor spectrum with USP6 gene rearrangement: multiple partners involved in the tissue repair process.Histology and histopathology · 2023Review
- Noncanonical Sonic Hedgehog signaling amplifies platelet reactivity and thrombogenicity.Blood advances · 2022Article
- The flagging features of the Sysmex XN-10 analyser for detecting platelet clumps and the impacts of platelet clumps on complete blood count parameters.Clinical chemistry and laboratory medicine · 2022Article
- Microelectromechanical System Measurement of Platelet Contraction: Direct Interrogation of Myosin Light Chain Phosphorylation.International journal of molecular sciences · 2021Article
- P2Y1 Purinergic Receptor Modulate Axon Initial Segment Initial Development.Frontiers in cellular neuroscience · 2019Article
- Agonist-Evoked Increases in Intra-Platelet Zinc Couple to Functional Responses.Thrombosis and haemostasis · 2019Article
- The role of integrin β1 in the heterogeneity of human embryonic stem cells culture.Biology open · 2018Article
- Activation of ROCK and MLCK tunes regional stress fiber formation and mechanics via preferential myosin light chain phosphorylation.Molecular biology of the cell · 2017Article
- Platelets stored at 4°C contribute to superior clot properties compared to current standard-of-care through fibrin-crosslinking.British journal of haematology · 2017Article
- New Concepts and Mechanisms of Platelet Activation Signaling.Physiology (Bethesda, Md.) · 2017Review
- Article
- Dissociation of SHP-1 from spinophilin during platelet activation exposes an inhibitory binding site for protein phosphatase-1 (PP1).PloS one · 2015Article
- Loss of ATE1-mediated arginylation leads to impaired platelet myosin phosphorylation, clot retraction, and in vivo thrombosis formation.Haematologica · 2014Article
- Platelets and cancer: a casual or causal relationship: revisited.Cancer metastasis reviews · 2014Review
- Trypsin causes platelet activation independently of known protease-activated receptors.Thrombosis and haemostasis · 2013Article
- Protease-activated receptor (PAR) 1 and PAR4 differentially regulate factor V expression from human platelets.Molecular pharmacology · 2013Article
- Rho associated coiled-coil kinase-1 regulates collagen-induced phosphatidylserine exposure in platelets.PloS one · 2013Article
- RhoA is essential for maintaining normal megakaryocyte ploidy and platelet generation.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMyosin IIA is an essential platelet contractile protein that is regulated by phosphorylation of its regulatory light chain (MLC) on residues (Thr)18 and (Ser)19 via the myosin light chain kinase (MLCK).
objectiveThe present study was carried out to elucidate the mechanisms regulating MLC (Ser)19 and (Thr)18 phosphorylation and the functional consequence of each phosphorylation event in platelets.
resultsInduction of 2MeSADP-induced shape change occurs within 5s along with robust phosphorylation of MLC (Ser)19 with minimal phosphorylation of MLC (Thr)18. Selective activation of G(12/13) produces both slow shape change and comparably slow MLC (Thr)18 and (Ser)19 phosphorylation. Stimulation with agonists that trigger ATP secretion caused rapid MLC (Ser)19 phosphorylation while MLC (Thr)18 phosphorylation was coincident with secretion. Platelets treated with p160(ROCK) inhibitor Y-27632 exhibited a partial inhibition in secretion and had a substantial inhibition in MLC (Thr)18 phosphorylation without effecting MLC (Ser)19 phosphorylation. These data suggest that phosphorylation of MLC (Ser)19 is downstream of Gq/Ca(2+) -dependent mechanisms and sufficient for shape change, whereas MLC (Thr)18 phosphorylation is substantially downstream of G(12/13) -regulated Rho kinase pathways and necessary, probably in concert with MLC (Ser)19 phosphorylation, for full contractile activity leading to dense granule secretion. Overall, we suggest that the amplitude of the platelet contractile response is differentially regulated by a least two different signaling pathways, which lead to different phosphorylation patterns of the myosin light chain, and this mechanism results in a graded response rather than a simple on/off switch.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.