ArticleJournal of neuroscience methods2010
A simple method of in vitro electroporation allows visualization, recording, and calcium imaging of local neuronal circuits.
Article in Journal of neuroscience methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.Nature communications · 2018Article
- Centrifugal Inputs to the Main Olfactory Bulb Revealed Through Whole Brain Circuit-Mapping.Frontiers in neuroanatomy · 2018Article
- Early Odorant Exposure Increases the Number of Mitral and Tufted Cells Associated with a Single Glomerulus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.Scientific reports · 2015Article
- In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation.Nature protocols · 2015Article
- The olfactory system as a model to study axonal growth patterns and morphology in vivo.Journal of visualized experiments : JoVE · 2014Article
- Distributed organization of a brain microcircuit analyzed by three-dimensional modeling: the olfactory bulb.Frontiers in computational neuroscience · 2014Article
- Time-lapse electrical recordings of single neurons from the mouse neocortex.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Activity regulates functional connectivity from the vomeronasal organ to the accessory olfactory bulb.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- High-throughput single-cell manipulation in brain tissue.PloS one · 2012Article
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Authors and funding
3 authors.
Funding
Abstract
Since Cajal's early drawings, the characterization of neuronal architecture has been paramount in understanding neuronal function. With the development of electrophysiological techniques that provide unprecedented access to the physiology of these cells, experimental questions of neuronal function have also become more tractable. Fluorescent tracers that can label the anatomy of individual or populations of neurons have opened the door to linking anatomy with physiology. Experimentally however, current techniques for bulk labeling of cells in vitro often affect neuronal function creating a barrier for exploring structure-function questions. Here we describe a new technique for highly localized electroporation within a cell or cell population that enables the introduction of membrane impermeable charged dyes including dextran-conjugated fluorophores, hydrazide tracers, and calcium indicator dyes in vitro. We demonstrate that this technique is highly versatile, allowing for labeling of large or small areas of tissue, allowing for the investigation of both cellular morphology and physiological activity in identified neuronal circuits in acute brain slices. Furthermore, this approach allows subsequent targeted whole-cell patch recording based on well-defined connectivity as well as assessment of physiological activity in targeted circuits on a fast time scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.