ArticleMarine biotechnology (New York, N.Y.)2011
Validation of housekeeping genes for gene expression studies in Symbiodinium exposed to thermal and light stress.
Article in Marine biotechnology (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 93 citations in OpenAlex.
- Article
- Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape.ISME communications · 2023Article
- Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress.Scientific reports · 2023Article
- Genome Mining as an Alternative Way for Screening the Marine Organisms for Their Potential to Produce UV-Absorbing Mycosporine-like Amino Acid.Marine drugs · 2022Review
- Algae from Aiptasia egesta are robust representations of Symbiodiniaceae in the free-living state.PeerJ · 2022Article
- Selection and evaluation of reference genes for qRT-PCR of Scutellaria baicalensis Georgi under different experimental conditions.Molecular biology reports · 2021Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Atypical Membrane-Anchored Cytokine MIF in a Marine Dinoflagellate.Microorganisms · 2020Article
- Genome Improvement and Core Gene Set Refinement ofMicroorganisms · 2020Article
- Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations.Nature communications · 2020Article
- Genome-Wide Analysis of Cell Cycle-Regulating Genes in the Symbiotic DinoflagellateG3 (Bethesda, Md.) · 2019Article
- Transcriptomic Responses to Thermal Stress and Varied Phosphorus Conditions inMicroorganisms · 2019Article
- A differential k-mer analysis pipeline for comparing RNA-Seq transcriptome and meta-transcriptome datasets without a reference.Functional & integrative genomics · 2019Article
- The role of chromatin dynamics under global warming response in the symbiotic coral model Aiptasia.Communications biology · 2019Article
- Article
- Validation of reference genes for cryopreservation studies with the gorgonian coral endosymbiont Symbiodinium.Scientific reports · 2017Article
- Enhancement of Non-photochemical Quenching as an Adaptive Strategy under Phosphorus Deprivation in the DinoflagellateFrontiers in microbiology · 2017Article
- Article
- Integral Light-Harvesting Complex Expression In Symbiodinium Within The Coral Acropora aspera Under Thermal Stress.Scientific reports · 2016Article
- Phosphorus Deficiency Inhibits Cell Division But Not Growth in the Dinoflagellate Amphidinium carterae.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unicellular photosynthetic algae (dinoflagellate) from the genus Symbiodinium live in mutualistic symbiosis with reef-building corals. Cultured Symbiodinium sp. (clade C) were exposed to a range of environmental stresses that included elevated temperatures (29°C and 32°C) under high (100 μmol quanta m(-2) s(-1) Photosynthetic Active Radiation) and low (10 μmol quanta m(-2) s(-1)) irradiances. Using real-time RT-PCR the stability of expression for the nine selected putative housekeeping genes (HKGs) was tested. The most stable expression pattern was identified for cyclophilin and S-adenosyl methionine synthetase (SAM) followed by S4 ribosomal protein (Rp-S4), Calmodulin (Cal), and Cytochrome oxidase subunit 1 (Cox), respectively. Thermal stress alone resulted in the highest expression stability for Rp-S4 and SAM, with a minimum of two reference genes required for data normalization. For Symbiodinium exposed to both, light and thermal stresses, at least five reference genes were recommended by geNorm analysis. In parallel, the expression of Hsp90 for Symbiodinium in culture and in symbiosis within coral host (Acropora millepora) was evaluated using the most stable HKGs. Our results revealed a drop in Hsp90 expression after an 18 h-period and a 24 h-period of exposure to elevated temperatures indicating the similar Hsp90 expression profile in symbiotic and non-symbiotic environments. This study provides the first list of the HKGs and will provide a useful reference in future gene expression studies in symbiotic dinoflagellates.
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