ArticlePloS one2010
Dynamic subunit exchange and the regulation of microtubule assembly by the stress response protein human alphaB crystallin.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- C-terminal extension of HSPB6 in a family with myopathy and cataract.Human molecular genetics · 2026Article
- Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.Frontiers in molecular neuroscience · 2021Article
- Small heat-shock proteins and their role in mechanical stress.Cell stress & chaperones · 2020Review
- Abnormal mitosis in reactive astrocytes.Acta neuropathologica communications · 2020Article
- Neuromuscular Diseases Due to Chaperone Mutations: A Review and Some New Results.International journal of molecular sciences · 2020Review
- Small heat shock proteins determine synapse number and neuronal activity during development.PloS one · 2020Article
- Using bicistronic constructs to evaluate the chaperone activities of heat shock proteins in cells.Scientific reports · 2017Article
- Desmin and αB-crystallin interplay in the maintenance of mitochondrial homeostasis and cardiomyocyte survival.Journal of cell science · 2016Article
- Heat shock proteins in the retina: Focus on HSP70 and alpha crystallins in ganglion cell survival.Progress in retinal and eye research · 2016Review
- Small Heat Shock Protein αB-Crystallin Controls Shape and Adhesion of Glioma and Myoblast Cells in the Absence of Stress.PloS one · 2016Article
- Microtubules and their role in cellular stress in cancer.Frontiers in oncology · 2014Review
- Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish.Developmental biology · 2013Article
- Chaperones: needed for both the good times and the bad times.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Chaperone-independent mitochondrial translocation and protection by αB-crystallin in RPE cells.Experimental eye research · 2013Article
- αB-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in lens and retinal cells.Biochimica et biophysica acta · 2012Article
- Small heat shock proteins in cellular adhesion and migration: evidence from Plasmodium genetics.Cell adhesion & migrationArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe small heat shock protein (sHSP), human alphaB crystallin, forms large, polydisperse complexes that modulate the tubulin-microtubule equilibrium using a dynamic mechanism that is poorly understood. The interactive sequences in alphaB crystallin for tubulin are surface exposed, and correspond to interactive sites for the formation of alphaB crystallin complexes. METHODOLOGY/PRINCIPAL
findingsThere is sequence homology between tubulin and the interactive domains in the beta8-strand of the core domain and the C-terminal extension of alphaB crystallin. This study investigated the hypothesis that the formation of tubulin and alphaB crystallin quaternary structures was regulated through common interactive domains that alter the dynamics of their assembly. Size exclusion chromatography (SEC), SDS-PAGE, microtubule assembly assays, aggregation assays, multiple sequence alignment, and molecular modeling characterized the dynamic response of tubulin assembly to increasing concentrations of alphaB crystallin. Low molar ratios of alphaB crystallin:tubulin were favorable for microtubule assembly and high molar ratios of alphaB crystallin:tubulin were unfavorable for microtubule assembly. Interactions between alphaB crystallin and unassembled tubulin were observed using SEC and SDS-PAGE. CONCLUSIONS/SIGNIFICANCE: Subunits of alphaB crystallin that exchange dynamically with the alphaB crystallin complex can interact with tubulin subunits to regulate the equilibrium between tubulin and microtubules.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.