ArticlePloS one2010
Role of NAD(P)H oxidase in superoxide generation and endothelial dysfunction in Goto-Kakizaki (GK) rats as a model of nonobese NIDDM.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Influence of a Hyperglycemic Microenvironment on a Diabetic Versus Healthy Rat Vascular Endothelium Reveals Distinguishable Mechanistic and Phenotypic Responses.Frontiers in physiology · 2019Article
- Effects of high-sodium intake on systemic blood pressure and vascular responses in spontaneously diabetic WBN/Kob-LeprClinical and experimental pharmacology & physiology · 2017Article
- Aging Increases Susceptibility to High Fat Diet-Induced Metabolic Syndrome in C57BL/6 Mice: Improvement in Glycemic and Lipid Profile after Antioxidant Therapy.Oxidative medicine and cellular longevity · 2016Article
- Boldine Ameliorates Vascular Oxidative Stress and Endothelial Dysfunction: Therapeutic Implication for Hypertension and Diabetes.Journal of cardiovascular pharmacology · 2015Review
- Contractile protein expression is upregulated by reactive oxygen species in aorta of Goto-Kakizaki rat.American journal of physiology. Heart and circulatory physiology · 2014Article
- Coronary endothelial dysfunction and mitochondrial reactive oxygen species in type 2 diabetic mice.American journal of physiology. Cell physiology · 2013Article
- Boldine improves endothelial function in diabetic db/db mice through inhibition of angiotensin II-mediated BMP4-oxidative stress cascade.British journal of pharmacology · 2013Article
- Type II diabetes increases mitochondrial DNA mutations in the left ventricle of the Goto-Kakizaki diabetic rat.American journal of physiology. Heart and circulatory physiology · 2013Article
- Cardiovascular changes in animal models of metabolic syndrome.Journal of diabetes research · 2013Article
- Glyoxalase 1 and glyoxalase 2 activities in blood and neuronal tissue samples from experimental animal models of obesity and type 2 diabetes mellitus.The journal of physiological sciences : JPS · 2012Article
- Targeting endothelial dysfunction in vascular complications associated with diabetes.International journal of vascular medicine · 2012Article
- Article
- Local oxidative and nitrosative stress increases in the microcirculation during leukocytes-endothelial cell interactions.PloS one · 2012Article
- Nicorandil prevents endothelial dysfunction due to antioxidative effects via normalisation of NADPH oxidase and nitric oxide synthase in streptozotocin diabetic rats.Cardiovascular diabetology · 2011Article
- Internal pudendal artery from type 2 diabetic female rats demonstrate elevated endothelin-1-mediated constriction.The journal of sexual medicine · 2011Article
- Rodent models for metabolic syndrome research.Journal of biomedicine & biotechnology · 2011Review
- The Contrary Impact Of Diabetes And Exercise On Endothelial Nitric Oxide Synthase Function.WebmedCentralArticle
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundCardiovascular disease is the leading cause of mortality in diabetics, and it has a complex etiology that operates on several levels. Endothelial dysfunction and increased generation of reactive oxygen species are believed to be an underlying cause of vascular dysfunction and coronary artery disease in diabetes. This impairment is likely the result of decreased bioavailability of nitric oxide (NO) within the vasculature. However, it is unclear whether hyperglycemia per se stimulates NADPH oxidase-derived superoxide generation in vascular tissue. METHODS AND
resultsThis study focused on whether NADPH oxidase-derived superoxide is elevated in vasculature tissue evoking endothelial/smooth muscle dysfunction in the hyperglycemic (169+/-4 mg%) Goto-Kakizaki (GK) rat. By dihydroethidine fluorescence staining, we determined that aorta superoxide levels were significantly elevated in 9 month-old GK compared with age matched Wistar (GK; 195+/-6%, Wistar; 100+/-3.5%). Consistent with these findings, 10(-6) mol/L acetylcholine-induced relaxation of the carotid artery was significantly reduced in GK rats compared with age matched Wistar (GK; 41+/-7%, Wistar; 100+/-5%) and measurements in the aorta showed a similar trend (p = .08). In contrast, relaxation to the NO donor SNAP was unaltered in GK compared to Wistar. Endothelial dysfunction was reversed by lowering of superoxide with apocynin, a specific Nox inhibitor.
conclusionsThe major findings from this study are that chronic hyperglycemia induces significant vascular dysfunction in both the aorta and small arteries. Hyperglycemic induced increases in NAD(P)H oxidase activity that did not come from an increase in the expression of the NAD(P)H oxidase subunits, but more likely as a result of chronic activation via intracellular signaling pathways.
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